In situ hybridization in light microscopy /

作者：  G鈋rard Morel, Annie Cavalier.

出版社：

简介：Summary: Publisher Summary 1 This volume is divided into eight chapters, each presenting one stage of the method: tissue preparation; pretreatment; hybridization itself; detection of the hybrid; and several methods for counterstaining and observation. Different types of probes and techniques for labeling them are analyzed and a chapter devoted to basic protocols is written to guide the newcomer through the correct methodology. Another chapter analyzes the controls, the problems of sensitivity, and the relationship between specific and nonspecific labeling. The method of preparation of different reagents and solutions, their sterilization, and their storage are described in the appendices, followed by a glossary of terms used. Annotation c. Book News, Inc., Portland, OR (booknews.com)  

In situ hybridization in electron microscopy /

作者：  G鈋rard Morel, Annie Cavalier, Lynda Williams.

出版社：

简介：Summary: Publisher Summary 1 Provides techniques and protocols for visualizing specific nucleic acid sequences in tissue and single-cell preparations through electron microscopy. Combines theory and practice, starting with basic principles, then breaks down the experimental process into successive steps illustrated by diagrams, protocols, and tables for choosing the right protocol. Information is given in parallel columns to convey practical comments and advice. Coverage encompasses tissue preparation, section preparation, pretreatment, hybridization, controls and problems, and protocol types. Separate appendices provide information on the preparation of equipment and reagents. For experienced and novice researchers. Morel is affiliated with the National Center of Scientific Research, France. Annotation c. Book News, Inc., Portland, OR (booknews.com)