Contents
Preface ...............................................................................................................
Contributors .......................................................................................................
Part I. Introduction to High Content Screening
1 Past, Present, and Future of High Content Screening
and the Field of Cellomics
D. Lansing Taylor .......................................................................... XXX
2 A Pharmaceutical Company User's Perspective
on the Potential of High Content Screening in Drug Discovery
Ann F. Hoffman and Ralph J. Garippa .......................................... XXX
3 Linking Microscopy and High Content Screening
in Large-Scale Biomedical Research
James G. Evans and Paul Matsudaira ............................................ XXX
Part II. Instrumentation, Biological Application Software, and Sample
Preparation
4 Requirements, Features, and Performance
of High-Content Screening Platforms
Albert H. Gough and Paul A. Johnston ......................................... XXX
5 Characteristics and Value of Directed Algorithms
in High Content Screening
Richik N. Ghosh, Oleg Lapets, and Jeffrey R. Haskins ................. XXX
6 Characteristics and Value of Machine Learning for Imaging
in High Content Screening
Juergen A. Klenk ........................................................................... XXX
7 Tools for Quantitative and Validated Measurements of Cells
Anne L. Plant, John T. Elliott, Alessandro Tona,
Dennis McDaniel, and Kurt J. Langenbach .............................. XXX
8 Automated Cell Plating and Sample Treatments for Fixed
Cells in High Content Assays
Gillian R. Richards, Julie E. Kerby, Grace K. Y. Chan,
and Peter B. Simpson ............................................................... XXX
9 Differentiating Primary Human Cells in Rapid-Throughput
Discovery Applications
viii Contents
Daniel R. Marshak and Dale E. Greenwalt ................................... XXX
10 Use of the CellCardTM System for Analyzing Multiple
Cell Types in Parallel
Oren Beske, Daniel Bassoni, and Simon Goldbard ...................... XXX
Part III. Reagents
11 Reagents to Measure and Manipulate Cell Functions
Kenneth A. Giuliano, D. Lansing Taylor,
and Alan S. Waggoner .............................................................. XXX
12 Fluorescent Proteins and Engineered Cell Lines
Nick Thomas ................................................................................. XXX
13 Optimizing the Integration of Immunoreagents
and Fluorescent Probes for Multiplexed
High Content Screening Assays
Kenneth A. Giuliano ..................................................................... XXX
14 The HaloTagTM: A Novel Technology for Cell Imaging
and Protein Analysis
Georgyi V. Los and Keith Wood ................................................... XXX
15 Protein Labeling With FlAsH and ReAsH
Thomas Machleidt, Matt Roberts,
and George Hanson .................................................................. XXX
16 Exploiting Network Biology to Improve Drug Discovery
Marnie L. MacDonald and John K. Westwick .............................. XXX
17 Physiological Indicators of Cell Function
Michael J. Ignatius and Jeffrey Hung ............................................ XXX
18 The Use of siRNA to Validate Immunofluorescence Studies
K. Gregory Moore, Wayne Speckmann,
and Ron Herzig ......................................................................... XXX
19 Caged Substrates Applied to High Content Screening:
An Introduction With an Eye to the Future
Peter G. Conrad, II, Rajesh V. Chavli,
and Richard S. Givens .............................................................. XXX
Part IV. Informatics and Bioinformatics
20 Overview of Informatics for High Content Screening
R. Terry Dunlay, Wallace J. Czekalski,
and Mark A. Collins .................................................................. XXX
21 Large-Scale Data Management for High Content Screening
Leon S. Garfinkel .......................................................................... XXX
22 An Integrated Biomedical Knowledge Extraction
and Analysis Platform: Using Federated Search
and Document Clustering Technology
Donald P. Taylor ........................................................................... XXX
23 Visualization of High Content Screening Data
Matthew J. Anstett ........................................................................ XXX
24 Pathway Mapping Tools for Analysis of High Content Data
Sean Ekins, Yuri Nikolsky, Andre Bugrim,
Eugene Kirillov, and Tatiana Nikolskaya .................................. XXX
Part V. Assays and Applications of High Content Screening
25 Systems Biology in Cancer Research: Genomics to Cellomics
Jackie L. Stilwell, Yinghui Guan, Richard M. Neve,
and Joe W. Gray ....................................................................... XXX
26 Target Validation in Drug Discovery
Robert A. Blake ............................................................................. XXX
27 High Content Screening as a Screening Tool in Drug Discovery
Anthony Nichols ........................................................................... XXX
28 Discovery of Protein Kinase Phosphatase Inhibitors
Andreas Vogt and John S. Lazo..................................................... XXX
29 High Content Translocation Assays for Pathway Profiling
Frosty Loechel, Sara Bjørn, Viggo Linde, Morten
Præstegaard, and Len Pagliaro ................................................. XXX
30 In Vitro Cytotoxicity Assessment
Peter O'Brien ................................................................................ XXX
31 Neurite Outgrowth in Retinal Ganglion Cell Culture
John B. Kerrison and Donald J. Zack ............................................ XXX
Index ..............................................................................................................