Morphology methods : cell and molecularbiology techniques /
副标题：无
作 者：edited by Ricardo V.Lloyd ; foreword by Ronald A. DeLellis.
分类号：
ISBN：9780896039551
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简介
Summary:
Publisher Summary 1
This reference for pathologists, histologists, anatomists, and cell biologists presents molecular and cell biologic techniques used in the in situ analysis of cells and tissues. Lloyd (Mayo Clinic) and other international researchers and clinicians describe techniques such as laser capture microdissection, Fluorescent In Situ Hybridization (FISH), confocal laser scanning microscopy, and tyramide amplification. Applications discussed include the diagnosis of endocrine and lymphoid lesions, the detection of infectious agents and DNA, and clonality analysis. The text is accompanied throughout by illustrations in black and white and color. Annotation c. Book News, Inc., Portland, OR (booknews.com)
Publisher Summary 2
Ricardo Lloyd, md, phd, provides critical and tutorial surveys of the cutting-edge molecular and cell biologic techniques used today in the analysis of cells and tissues in situ. These molecular-based histologic and morphologic techniques are described in step-by-step detail by hands-on experts, often those who have originated or perfected them. Among the techniques presented are laser capture microdissection, in situ hybridization, FISH, confocal laser scanning microscopy, in situ PCR, and tyramide amplification. Applications include the detection of infectious agents and DNA, the diagnosis of undifferentiated tumors and endocrine lesions, the metaphase analysis of whole blood cultures, and clonality analysis. In its integrated, state-of-the-art approach, Morphology Methods: Cell and Molecular Biology Techniques thoroughly details and applies the rich panoply of significant new cell and molecular biologic technologies to all the major problems confronting morphologic studies and diagnostic pathology today.
目录
Foreword p. v
Preface p. vii
Contributors p. xiii
Color Plates p. xv
1 Introduction to Molecular Methods Ricardo V. Lloyd p. 1
2 Laser Capture Microdissection: Principles and Applications Ricardo V. Lloyd p. 11
Protocol: HandE Staining p. 23
Protocol: Operating the PIX Cell System p. 24
Protocol: DNA Extraction from Collected Cells p. 24
Protocol: RNA Extraction from Collected Cells p. 25
3 In Situ Hybridization: Detection of DNA and RNA Long Jin and Xiang Qian and Ricardo V. Lloyd p. 27
Protocol: In Situ Hybridization p. 45
4 In Situ Detection of Infectious Agents Gary W. Procop and Randall Hayden p. 47
Protocol: Detecting Adenovirus p. 61
Protocol: Epstein-Barr Virus (Eber I/II) p. 63
5 Fluorescent In Situ Hybridization Susan Sheldon p. 67
Protocol: FISH p. 84
6 Practical Applications of the FISH Technique George Kontogeorgos and Nikiforos Kapranos and Eleni Thodou p. 91
Protocol: Metaphase Analysis from Whole Blood Culture p. 102
Protocol: Metaphase Analysis from Solid Tumors p. 106
Protocol: Interphase Analysis on Cell Imprints--Cytology Specimens p. 108
Protocol: Interphase Analysis of Formalin-Fixed Paraffin-Embedded Tissues p. 110
7 Tyramide Amplification Methods for In Situ Hybridization John L. Frater and Raymond R. Tubbs p. 113
Protocol: TSA by Rapp et al., 1995 p. 125
Protocol: CARD/TSA with Nanogold p. 126
Protocol: Tyramide-enhanced FISH with Peroxidase-labeled Oligonucleotides p. 127
8 Protocols: Ultrastructural In Situ Hybridization Akira Matsuno and Tadashi Nagashima and R. Yoshiyuki Osamura and Keiichi Watanabe p. 129
9 Quantitation of In Situ Hybridization Analysis Lars-Inge Larsson p. 145
10 Protocols: Confocal Laser Scanning Microscopy Akira Matsuno and Johbu Itoh and Tadashi Nagashima and R. Yoshiyuki Osamura and Keiichi Watanabe p. 165
11 Protocols: Polymerase Chain Reaction Yuri E. Nikiforov and Philip N. Howles p. 181
12 Protocols: Detection of Nucleic Acids in Cells and Tissues by In Situ Polymerase Chain Reaction Omar Bagasra and Lisa E. Bobroski and Muhammad Amjad p. 209
13 Immunohistochemistry: Theory and Practice Patrick C. Roche and Eric D. Hsi p. 229
14 Antigen Retrieval Technique for Immunohistochemistry: Principles, Protocols, and Further Development Clive R. Taylor and Shan-Rong Shi p. 239
Protocol: Antigen Retrieval (AR) Technique p. 260
15 Tyramide Amplification in Immunohistochemistry Naoko Sanno and Akira Teramoto and R. Yoshiyuki Osamura p. 267
Protocol: CARD Immunohistochemistry p. 276
16 Application of Immunohistochemistry in the Diagnosis of Lymphoid Lesions Paul J. Kurtin p. 277
17 Applications of Immunohistochemistry in the Diagnosis of Undifferentiated Tumors Mark R. Wick and Lisa A. Cerilli p. 323
18 Applications of Immunohistochemistry in the Diagnosis of Endocrine Lesions Ricardo V. Lloyd p. 361
19 Ultrastructural Immunohistochemistry Sergio Vidal and Eva Horvath and Kalman Kovacs p. 375
Protocol: Ultrastructural Immunohistochemistry p. 399
20 Protocols: Clonality Analysis Aurel Perren and Paul Komminoth p. 403
Index p. 413
Preface p. vii
Contributors p. xiii
Color Plates p. xv
1 Introduction to Molecular Methods Ricardo V. Lloyd p. 1
2 Laser Capture Microdissection: Principles and Applications Ricardo V. Lloyd p. 11
Protocol: HandE Staining p. 23
Protocol: Operating the PIX Cell System p. 24
Protocol: DNA Extraction from Collected Cells p. 24
Protocol: RNA Extraction from Collected Cells p. 25
3 In Situ Hybridization: Detection of DNA and RNA Long Jin and Xiang Qian and Ricardo V. Lloyd p. 27
Protocol: In Situ Hybridization p. 45
4 In Situ Detection of Infectious Agents Gary W. Procop and Randall Hayden p. 47
Protocol: Detecting Adenovirus p. 61
Protocol: Epstein-Barr Virus (Eber I/II) p. 63
5 Fluorescent In Situ Hybridization Susan Sheldon p. 67
Protocol: FISH p. 84
6 Practical Applications of the FISH Technique George Kontogeorgos and Nikiforos Kapranos and Eleni Thodou p. 91
Protocol: Metaphase Analysis from Whole Blood Culture p. 102
Protocol: Metaphase Analysis from Solid Tumors p. 106
Protocol: Interphase Analysis on Cell Imprints--Cytology Specimens p. 108
Protocol: Interphase Analysis of Formalin-Fixed Paraffin-Embedded Tissues p. 110
7 Tyramide Amplification Methods for In Situ Hybridization John L. Frater and Raymond R. Tubbs p. 113
Protocol: TSA by Rapp et al., 1995 p. 125
Protocol: CARD/TSA with Nanogold p. 126
Protocol: Tyramide-enhanced FISH with Peroxidase-labeled Oligonucleotides p. 127
8 Protocols: Ultrastructural In Situ Hybridization Akira Matsuno and Tadashi Nagashima and R. Yoshiyuki Osamura and Keiichi Watanabe p. 129
9 Quantitation of In Situ Hybridization Analysis Lars-Inge Larsson p. 145
10 Protocols: Confocal Laser Scanning Microscopy Akira Matsuno and Johbu Itoh and Tadashi Nagashima and R. Yoshiyuki Osamura and Keiichi Watanabe p. 165
11 Protocols: Polymerase Chain Reaction Yuri E. Nikiforov and Philip N. Howles p. 181
12 Protocols: Detection of Nucleic Acids in Cells and Tissues by In Situ Polymerase Chain Reaction Omar Bagasra and Lisa E. Bobroski and Muhammad Amjad p. 209
13 Immunohistochemistry: Theory and Practice Patrick C. Roche and Eric D. Hsi p. 229
14 Antigen Retrieval Technique for Immunohistochemistry: Principles, Protocols, and Further Development Clive R. Taylor and Shan-Rong Shi p. 239
Protocol: Antigen Retrieval (AR) Technique p. 260
15 Tyramide Amplification in Immunohistochemistry Naoko Sanno and Akira Teramoto and R. Yoshiyuki Osamura p. 267
Protocol: CARD Immunohistochemistry p. 276
16 Application of Immunohistochemistry in the Diagnosis of Lymphoid Lesions Paul J. Kurtin p. 277
17 Applications of Immunohistochemistry in the Diagnosis of Undifferentiated Tumors Mark R. Wick and Lisa A. Cerilli p. 323
18 Applications of Immunohistochemistry in the Diagnosis of Endocrine Lesions Ricardo V. Lloyd p. 361
19 Ultrastructural Immunohistochemistry Sergio Vidal and Eva Horvath and Kalman Kovacs p. 375
Protocol: Ultrastructural Immunohistochemistry p. 399
20 Protocols: Clonality Analysis Aurel Perren and Paul Komminoth p. 403
Index p. 413
Morphology methods : cell and molecularbiology techniques /
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