简介
Covers biophysical, molecular biological, and cell biological techniques involved in the study of calcium signaling. Examines a range of topics, including techniques for imaging and measuring calcium in subcellular compartments and patch clamp investigation of calcium channels, and addresses special problems associated with calcium signaling systems. Serves as a reference for scientists studying specific biological processes and systems, and those studying the cellular and molecular basis of diseases. The editor is chief of the Calcium Regulation Section at the National Institute of Environmental Health Sciences at the National Institutes of Health. Annotation c. Book News, Inc., Portland, OR (booknews.com)
目录
Chapter 1 Fluorescent Calcium Indicators Based on BAPTA Martin Poenie p. 1
Chapter 2 Techniques for Imaging and Measuring Calcium in Subcellular Compartments Aldebaran M. Hofer and Tullio Pozzan p. 47
Chapter 3 Subcellular Fractionation and Intracellular Calcium Stores Jan B. Parys and Sara Vanlingen and Luc Raeymaekers and Humbert De Smedt and Ludwig Missiaen p. 71
Chapter 4 Measuring Ca[superscript 2+] Fluxes in Permeabilized Cells Colin W. Taylor p. 111
Chapter 5 Patch-Clamp Investigation of Calcium Channels David L. Armstrong and Lisa M. Broad p. 131
Chapter 6 Xenopus Oocytes as a Tool in Calcium Signaling Research Patricia Camacho and James D. Lechleiter p. 157
Chapter 7 Inositol 1,4,5-Trisphosphate Receptor: Functional Aspects Jean-Francois Coquil and Laurent Picard and Jean-Pierre Mauger p. 183
Chapter 8 Inositol 1,4,5-Trisphosphate Receptors: Molecular Aspects Suresh K. Joseph and Darren Boehning and Chi Lin p. 203
Chapter 9 Ryanodine Receptors Steven O. Marx and Andrew R. Marks p. 227
Chapter 10 Methods in Cyclic ADP-Ribose and NAADP Research Antony Galione and Jose-Manuel Cancela and Grant C. Churchill and Armando Genazzani and Chetan Lad and Justyn Thomas and Heather Wilson and Derek Terrar p. 249
Chapter 11 Molecular Mechanisms by Which Ca[superscript 2+]/Calmodulin Recognizes, Binds, and Activates Calmodulin-Dependent Protein Kinases David Chin p. 297
Chapter 12 Molecular Biology of Calcium Channels Stephan Philipp and Ulrich Wissenbach and Veit Flockerzi p. 321
Chapter 13 Measuring Single Cell and Subcellular Ca[superscript 2+] Signals Sandip Patel and Lawrence D. Robb-Gaspers and Andrew P. Thomas p. 343
Chapter 14 Methods for Studying Calcium Control of Secretion Oliver Nu[sz ligature]e and Lena Serrander and Karl-Heinz Krause p. 365
Index p. 391
Chapter 2 Techniques for Imaging and Measuring Calcium in Subcellular Compartments Aldebaran M. Hofer and Tullio Pozzan p. 47
Chapter 3 Subcellular Fractionation and Intracellular Calcium Stores Jan B. Parys and Sara Vanlingen and Luc Raeymaekers and Humbert De Smedt and Ludwig Missiaen p. 71
Chapter 4 Measuring Ca[superscript 2+] Fluxes in Permeabilized Cells Colin W. Taylor p. 111
Chapter 5 Patch-Clamp Investigation of Calcium Channels David L. Armstrong and Lisa M. Broad p. 131
Chapter 6 Xenopus Oocytes as a Tool in Calcium Signaling Research Patricia Camacho and James D. Lechleiter p. 157
Chapter 7 Inositol 1,4,5-Trisphosphate Receptor: Functional Aspects Jean-Francois Coquil and Laurent Picard and Jean-Pierre Mauger p. 183
Chapter 8 Inositol 1,4,5-Trisphosphate Receptors: Molecular Aspects Suresh K. Joseph and Darren Boehning and Chi Lin p. 203
Chapter 9 Ryanodine Receptors Steven O. Marx and Andrew R. Marks p. 227
Chapter 10 Methods in Cyclic ADP-Ribose and NAADP Research Antony Galione and Jose-Manuel Cancela and Grant C. Churchill and Armando Genazzani and Chetan Lad and Justyn Thomas and Heather Wilson and Derek Terrar p. 249
Chapter 11 Molecular Mechanisms by Which Ca[superscript 2+]/Calmodulin Recognizes, Binds, and Activates Calmodulin-Dependent Protein Kinases David Chin p. 297
Chapter 12 Molecular Biology of Calcium Channels Stephan Philipp and Ulrich Wissenbach and Veit Flockerzi p. 321
Chapter 13 Measuring Single Cell and Subcellular Ca[superscript 2+] Signals Sandip Patel and Lawrence D. Robb-Gaspers and Andrew P. Thomas p. 343
Chapter 14 Methods for Studying Calcium Control of Secretion Oliver Nu[sz ligature]e and Lena Serrander and Karl-Heinz Krause p. 365
Index p. 391
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