HPLC of peptides and proteins : methods and protocols /

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作者：edited by Marie-Isabel Aguilar.

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ISBN：9780896039773

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简介

简介

Summary: Publisher Summary 1 International experts from academia and industry offer protocols for performing commonly used techniques of high-performance liquid chromatography (HPLC), which have revolutionized the characterization of basic biological processes and development of novel peptide and protein-based pharmaceuticals. Following an overview of basic HPLC theory and methodology, Aguilar (biochemistry and molecular biology, Monash U., Clayton, Victoria, Australia) introduces the 27 chapters' themes, including achieving high resolution and capillary to large-scale preparative isolation. Case studies illustrate analytical challenges and diverse applications in biochemistry and biotechnology. Annotation 漏2004 Book News, Inc., Portland, OR (booknews.com) Publisher Summary 2 Hands-on experts from academia and industry comprehensively describe how to successfully perform all the critical HPLC techniques needed for the analysis of peptides and proteins. The methods range from commonly used techniques to those for capillary to large-scale preparative isolation. The authors have also presented a number of specific applications as case studies to illustrate the analytical approaches to a particular separation or assay challenge, with examples drawn from contemporary fields in biochemistry and biotechnology.

Contents
Preface
Contributors
I. MODES OF HPLC
1 HPLC of Peptides and Proteins: Basic Theory and Methodology
Marie-Isabel Aguilar
2 Reversed Phase High Performance Liquid Chromatography
Marie-Isabel Aguilar
3 Ion-Exchange Chromatography
Peter Stanton
4 High Performance Hydrophobic Interaction Chromatography
Kçlmçn Benedek
5 Gel Filtration Chromatography
Peter Stanton
6 Hydrophilic Interaction Chromatography
Herbert Lindner and Wilfried Helliger
7 Immobilized Metal Ion Affinity Chromatography of Proteins
Michael Zachariou
8 Immunoaffinity Chromatography of Proteins
Stuart Gallant
9 Liquid Chromatography: Mass Spectrometry and Tandem Mass Spectrometry of Peptides and Proteins
Tun-Li Shen and Kathleen R. Noon
II. PREPARATIVE METHODOLOGIES
10 Capillary Separations
Montse Carrascal and Joaquin Abian
11 Micropreparative HPLC of Peptides and Proteins
Eduoard C. Nice and Marie-Isabel Aguilar
12 Multidimensional HPLC Purification of Proteins
Eduoard C. Nice and Marie-Isabel Aguilar
13 Analytical High Performance Liquid Chromatography
Kçlmçn Benedek
14 Prep/Semi-prep Separations of Peptides
Denis B. Scanlon and James Finlayson
15 Large Scale Protein Chromatography
Joseph Bertolini, Peter Gomme, and Patrick Thomas
III. APPLICATIONS
16 Proteolytic Peptide Mapping
Peter Hojrup
17 Mass Spectrometric Characterization of Post-Translationally
Modified Proteins-Phosphorylation
Martin R. Larsen
18 Analyses of Glycopeptides and Glycoproteins by Liquid Chromatography /Mass Spectrometry and Liquid
Chromatography/Tandem Mass Spectrometry
Nana Kawasaki, Miyako Ohta, Satsuki Itoh,
and Takao Hayakawa
19 The Use of HPLC in the Analysis of Peptide Metabolism
Rebecca A. Lew
20 Isolation and Characterization of Naturally Processed MHC-Bound Peptides from the Surface of Antigen Presenting Cells
Anthony W. Purcell
21 Venom Analysis of Australian Funnel-Web Spiders (Atrax Sp.)
Using On-Line Reversed Phase High Performance Liquid Chromatography/Electrospray Ionization Mass Spectrometry Techniques
David Wilson and Paul Alewood
22 HPLC and Mass Spectrometry of Intrinsic Membrane Proteins
Julian P. Whitelegge
23 IgG Purification
Maree S. Powell and Bruce D. Wines
24 DNA Binding Proteins: Use of LC-MS to Identify Key Domains
in RNA Polymerase-Promoter Interactions
Malcolm Buckle
25 Sensitive Enzymatic Analysis of Histidine Decarboxylase
Using HPLC
Jordi Gùmez-Ramirez, Isaac Blanco, and Jordi Ortiz
26 Automated Versus Manual Peptide Libraries Profiling
by Mass Spectrometry
Jean-Louis Aubagnac, Christine Enjalbal, Jean Martinez,
Pierre Sanchez, and Gilles Subra
27 Proteome Analysis
L. Matthew Powell and Aaron T. Timperman
Index

Library of Congress Subject Headings for this publication: Peptides Analysis, Proteins Analysis, High performance liquid chromatography, Peptides chemistry, Chromatography, High Pressure Liquid methods, Proteins chemistry

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