MicroRNA protocols /
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ISBN：9781588295811
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Summary:
Publisher Summary 1
MicroRNAs are native small regulatory RNAs of about 22 nucleotides in eukaryotes, evolutionarily conserved molecules that can repress translation or degrade the RNA transcripts of target genes, depending on the degree of complementarity. According to Ying (neurobiology, Keck School of Medicine, U. of Southern California) they are useful for the design of new therapies against cancer polymorphism and viral mutation that are more advantageous than those derived from short interfering RNAs in that microRNAs are long-acting, stable in vivo, highly RNA promoter- compatible, multiple targeting, and of on overt toxicity. The main goal of this work is to present a range of descriptions of microRNAs in such species as worms, flies, fish, chicks, mice, and humans, as well as adaptations and applications of interest to members of the research community already familiar with microRNAs. Twenty-six papers examine such topics as structure and analysis of microRNA precursors, recognition and cleavage of primary microRNA transcripts, the microRNA sequence database, in situ hybridization as a tool to study the role of microRNAs in plant development, cloning microRNAs from mammalian tissues, and gene silencing in vitro and in vivo using intronic microRNAs, to name a few examples. Annotation 漏2006 Book News, Inc., Portland, OR (booknews.com)
Publisher Summary 2
MicroRNA Protocols provides diverse, novel, and useful descriptions of miRNAs in several species, including plants, worms, flies, fish, chicks, mice, and humans. These include some useful adaptations and applications that could be relevant to the wider research community who are already familiar with the identification of miRNAs. This volume will stimulate the reader to explore diverse ways to understanding the mechanism in which miRNAs facilitate the molecular aspects of the biomedical research.
目录
Preface p. v
Contributors p. xi
1 The MicroRNA: Overview of the RNA Gene That Modulates Gene Functions Shao-Yao Ying and Donald C. Chang and Joseph D. Miller and Shi-Lung Lin p. 1
2 Structure Analysis of MicroRNA Precursors Jacek Krol and Wlodzimierz J. Krzyzosiak p. 19
3 MicroRNA Biogenesis: Isolation and Characterization of the Microprocessor Complex Richard I. Gregory and Thimmaiah P. Chendrimada and Ramin Shiekhatter p. 33
4 Recognition and Cleavage of Primary MicroRNA Transcripts Yan Zeng and Bryan R. Cullen p. 49
5 Mouse Embryonic Stem Cells as a Model Genetic System to Dissect and Exploit the RNA Interference Machinery Stefan A. Muljo and Chryssa Kanellopoulou p. 57
6 MicroRNAs and Messenger RNA Turnover Julia A. Chekanova and Dmitry A. Belostotsky p. 73
7 Prediction of MicroRNA Targets Marc Rehmsmeier p. 87
8 Prediction of Human MicroRNA Targets Bino John and Chris Sander and Debora S. Marks p. 101
9 Complications in Mammalian MicroRNA Target Prediction Neil R. Smalheiser and Vetle I. Torvik p. 115
10 miRBase: The MicroRNA Sequence Database Sam Griffiths-Jones p. 129
11 Methodologies for High-Throughput Expression Profiling of MicroRNAs Paz Einat p. 139
12 In Situ Hybridization as a Tool to Study the Role of MicroRNAs in Plant Development Catherine Kidner and Marja Timmermans p. 159
13 Usefulness of the Luciferase Reporter System to Test the Efficacy of siRNA Fengfeng Zhuang and Yi-Hsin Liu p. 181
14 Cloning MicroRNAs From Mammalian Tissues Michael Z. Michael p. 189
15 Methods for Analyzing MicroRNA Expression and Function During Hematopoietic Lineage Differentiation Hyeyoung Min and Chang-Zheng Chen p. 209
16 Identifying MicroRNA Regulators of Cell Death in Drosophila Chun-Hong Chen and Ming Guo and Bruce A. Hay p. 229
17 MicroRNAs in Human Immunodeficiency Virus-1 Infection Yamina Bennasser and Shu-Yun Le and Man Lung Yeung and Kuan-Teh Jeang p. 241
18 Cloning and Detection of HIV-1-Encoded MicroRNA Shinya Omoto and Yoichi R. Fujii p. 255
19 Identification of Messenger RNAs and MicroRNAs Associated With Fragile X Mental Retardation Protein Ranhui Duan and Peng Jin p. 267
20 In Vitro Precursor MicroRNA Processing Assays Using Drosophila Schneider-2 Cell Lysates Akira Ishizuka and Kuniaki Saito and Mikiko C. Siomi and Haruhiko Siomi p. 277
21 Downregulation of Human Cdc6 Protein Using a Lentivirus RNA Interference Expression Vector Feng Luo and Jiing-Kuan Yee and Sheng-He Huang and Ling-Tao Wu and Ambrose Y. Jong p. 287
22 Gene Silencing In Vitro and In Vivo Using Intronic MicroRNAs Shi-Lung Lin and Shao-Yao Ying p. 295
23 Isolation and Identification of Gene-Specific MicroRNAs Shi-Lung Lin and Donald C. Chang and Shao-Yao Ying p. 313
24 Transgene-Like Animal Models Using Intronic MicroRNAs Shi-Lung Lin and Shin-Ju E. Chang and Shao-Yao Ying p. 321
25 Evolution of MicroRNAs Andrea Tanzer and Peter F. Stadler p. 335
26 Perspectives Shao-Yao Ying and Donald C. Chang and Joseph D. Miller and Shi-Lung Lin p. 351
Index p. 359
Contributors p. xi
1 The MicroRNA: Overview of the RNA Gene That Modulates Gene Functions Shao-Yao Ying and Donald C. Chang and Joseph D. Miller and Shi-Lung Lin p. 1
2 Structure Analysis of MicroRNA Precursors Jacek Krol and Wlodzimierz J. Krzyzosiak p. 19
3 MicroRNA Biogenesis: Isolation and Characterization of the Microprocessor Complex Richard I. Gregory and Thimmaiah P. Chendrimada and Ramin Shiekhatter p. 33
4 Recognition and Cleavage of Primary MicroRNA Transcripts Yan Zeng and Bryan R. Cullen p. 49
5 Mouse Embryonic Stem Cells as a Model Genetic System to Dissect and Exploit the RNA Interference Machinery Stefan A. Muljo and Chryssa Kanellopoulou p. 57
6 MicroRNAs and Messenger RNA Turnover Julia A. Chekanova and Dmitry A. Belostotsky p. 73
7 Prediction of MicroRNA Targets Marc Rehmsmeier p. 87
8 Prediction of Human MicroRNA Targets Bino John and Chris Sander and Debora S. Marks p. 101
9 Complications in Mammalian MicroRNA Target Prediction Neil R. Smalheiser and Vetle I. Torvik p. 115
10 miRBase: The MicroRNA Sequence Database Sam Griffiths-Jones p. 129
11 Methodologies for High-Throughput Expression Profiling of MicroRNAs Paz Einat p. 139
12 In Situ Hybridization as a Tool to Study the Role of MicroRNAs in Plant Development Catherine Kidner and Marja Timmermans p. 159
13 Usefulness of the Luciferase Reporter System to Test the Efficacy of siRNA Fengfeng Zhuang and Yi-Hsin Liu p. 181
14 Cloning MicroRNAs From Mammalian Tissues Michael Z. Michael p. 189
15 Methods for Analyzing MicroRNA Expression and Function During Hematopoietic Lineage Differentiation Hyeyoung Min and Chang-Zheng Chen p. 209
16 Identifying MicroRNA Regulators of Cell Death in Drosophila Chun-Hong Chen and Ming Guo and Bruce A. Hay p. 229
17 MicroRNAs in Human Immunodeficiency Virus-1 Infection Yamina Bennasser and Shu-Yun Le and Man Lung Yeung and Kuan-Teh Jeang p. 241
18 Cloning and Detection of HIV-1-Encoded MicroRNA Shinya Omoto and Yoichi R. Fujii p. 255
19 Identification of Messenger RNAs and MicroRNAs Associated With Fragile X Mental Retardation Protein Ranhui Duan and Peng Jin p. 267
20 In Vitro Precursor MicroRNA Processing Assays Using Drosophila Schneider-2 Cell Lysates Akira Ishizuka and Kuniaki Saito and Mikiko C. Siomi and Haruhiko Siomi p. 277
21 Downregulation of Human Cdc6 Protein Using a Lentivirus RNA Interference Expression Vector Feng Luo and Jiing-Kuan Yee and Sheng-He Huang and Ling-Tao Wu and Ambrose Y. Jong p. 287
22 Gene Silencing In Vitro and In Vivo Using Intronic MicroRNAs Shi-Lung Lin and Shao-Yao Ying p. 295
23 Isolation and Identification of Gene-Specific MicroRNAs Shi-Lung Lin and Donald C. Chang and Shao-Yao Ying p. 313
24 Transgene-Like Animal Models Using Intronic MicroRNAs Shi-Lung Lin and Shin-Ju E. Chang and Shao-Yao Ying p. 321
25 Evolution of MicroRNAs Andrea Tanzer and Peter F. Stadler p. 335
26 Perspectives Shao-Yao Ying and Donald C. Chang and Joseph D. Miller and Shi-Lung Lin p. 351
Index p. 359
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