Testing of genetically modified organisms in foods /

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作   者:Farid E. Ahmed, editor.

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ISBN:9781560222743

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简介

Ten American and European academics and researchers contribute ten chapters covering the areas of risks and benefits of GMOs in the food supply and environment, sampling concepts and plans in both the EU and the U.S., reference material and standards, protein-based methods and DNA-based methods of testing, near-infrared spectroscopy, and other methods that may be of academic/research value or applicable to field testing. For students in food safety courses, food technologists and researchers, food handlers and suppliers, quality control/assurance staff, and scientists and administrators working in the regulatory arena. Annotation 漏2004 Book News, Inc., Portland, OR (booknews.com)

目录

About the Editor p. ix
Contributors p. xi
Preface p. xiii
Chapter 1. Risks and Benefits of Agricultural Biotechnology Anthony M. Shelton p. 1
The Development of Biotechnology p. 1
Beginning of the Controversy About Biotechnology p. 5
Risk and Benefit Analysis p. 6
The Present Scope of Agricultural Biotechnology Products p. 7
Regulations on Growing Biotechnology Crops p. 10
Grower Adoption of Biotechnology Crops p. 13
Economics of Using Current Biotechnology Crops p. 14
The Concept of Substantial Equivalence and Its Impact on Product Liability and International Trade p. 17
Food Safety Issues of Biotechnology Products p. 22
Framework for Evaluating Environmental Effects of Current Biotechnology Crops p. 26
Gene Flow in Transgenic Crops p. 28
Gene Containment Strategies for Biotechnology Crops p. 30
Economic Consequences of Gene Flow from Biotechnology Crops p. 31
Effects of Biotechnology Crops on Nontarget Organisms p. 32
Environmental Consequences of Two Novel GM Organisms p. 36
Pesticide-Use Patterns p. 38
Resistance Management to Herbicide-Resistant and Insect-Resistant Plants p. 42
Agricultural Biotechnology in a Changing World p. 44
Chapter 2. Sampling for the Detection of Biotech Grains: A USDA Perspective Larry D. Freese p. 55
Introduction p. 55
Introduction to Sampling Theory p. 57
Conclusions p. 74
Chapter 3. Sampling for GMO Analysis: The European Perspective Claudia Paoletti p. 77
Introduction p. 77
The Concept of Sampling and Currently Adopted Sampling Protocols p. 82
Limitations of the Currently Adopted Sampling Protocols p. 90
Sampling for GMO Detection/Quantification: Recommendations p. 94
Concluding Remarks p. 96
Chapter 4. Reference Materials and Standards Stefanie Trapmann and Philippe Corbisier and Heinz Schimmel p. 101
Introduction p. 101
Matrix-Based GMO CRMs p. 102
Pure Genomic DNA GMO CRMs p. 109
Pure Plasmid DNA GMO CRMs p. 109
Pure Protein GMO CRMs p. 111
Discussion p. 111
Chapter 5. Protein-Based Methods: Elucidation of the Principles Farid E. Ahmed p. 117
Introduction p. 117
Problems Associated with Developing Immunoassays for Food Proteins and Suggested Remedies p. 121
Western Blotting p. 129
Immunoassays p. 133
Conclusions p. 141
Chapter 6. Protein-Based Methods: Case Studies James W. Stave p. 147
Introduction p. 147
Foods: Processed and Finished p. 147
Grain and Seed p. 152
Discussion p. 158
Chapter 7. DNA-Based Methods for Detection and Quantification of GMOs: Principles and Standards John Fagan p. 163
Introduction p. 163
The Polymerase Chain Reaction p. 165
The Future of GMO Testing p. 210
Near-Infrared Spectroscopy p. 212
DNA Microarrays p. 213
Biosensors p. 215
Chapter 8. DNA-Based Methods for GMO Detection: Historical Developments and Future Prospects Farid E. Ahmed p. 221
Southern Hybridization/Slot or Dot Blotting Techniques p. 221
Polymerase Chain Reaction Applications p. 226
Gene Expression and Microarray Technology p. 232
DNA-Based Biosensors p. 238
Conclusions p. 248
Chapter 9. Near-Infrared Spectroscopic Methods Sylvie A. Roussel and Robert P. Cogdill p. 255
Introduction: The Limitations of Applying Current GMO Screening Methods p. 255
Introduction to Near-Infrared Spectroscopy p. 258
Roundup Ready Soybean Detection Case Study p. 266
Quality Assurance Issues p. 275
Future Considerations p. 279
Chapter 10. Other Methods for GMO Detection and Overall Assessment of the Risks Farid E. Ahmed p. 285
Metabolic Profiling p. 285
Proteomics p. 286
Green Fluorescent Protein As a Marker in Transgenic Plants p. 293
Concerns About Gene Flow, Liabilities, Regulatory Climates, and Intellectual Property Rights p. 295
Assessment of Safety Issues p. 299
Conclusions p. 307
Index p. 315

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