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ISBN:9781588297440

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Summary: Publisher Summary 1 Because stem cells can grow and divide indefinitely and can also differentiate into specialized cell types, they promise significant advances in regenerative medicine. Recent work has led to the development of new methods for embryonic and adult stem cell isolation, characterization, expansion in undifferentiated state, customized differentiation, efficient stem cell transduction for gene therapy, quick monitoring of rejection, and other advances. This volume, edited by Vemuri (Invitrogen Corporation), presents a sample of methodological advances for wider use by novice and expert scientists, including derivation of human embryonic stem cells in xeno-free conditions, digital imaging of stem cells by electron microscopy, controlled-cooling protocol for cryopreservation of human and non-human primate embryonic stem cells, cell surface markers in human embryonic stem cells, generation of a monoclonal antibody library against human embryonic stem cells, gene transfer via nucleofection into adult and embryonic stem cells, isolation of stem cells from human umbilical cord blood in serum-free conditions, hematopoietic colony-forming cell assays, assays for alloreactive responses by polymerase chain reaction, adenoviral transduction of mesechymal stem cells, and 13 others. As in the rest of the series, each chapter describes laboratory protocols, necessary materials, troubleshooting, and known pitfalls. Annotation 漏2008 Book News, Inc., Portland, OR (booknews.com)   Publisher Summary 2 The scope for improving health care using stem cell therapies is thrilling, but has considerable technical challenges and methodological constraints that need to be addressed. Keeping with the tradition of Humana Press to bring these developments to the forefront in a timely manner, this book presents scientific advances in stem cell methods for a wider use by novice and expert scientists, through the series of Methods in Molecular Biology.  

目录

Table Of Contents:
Preface v
Contributors xi

Derivation of Human Embryonic Stem Cells in Xeno-Free Conditions 1(10)

Mohan C. Vemuri

Tim Schimmel

Pere Colls

Santiago Munne

Jacques Cohen

Feeder-Layer Free Culture System for Human Embryonic Stem Cells 11(10)

Michal Amit

Digital Imaging of Stem Cells by Electron Microscopy 21(22)

A. Henry Sathananthan

Stefania A. Nottola

A Controlled-Cooling Protocol for Cryopreservation of Human and Non-Human Primate Embryonic Stem Cells 43(8)

Carol B. Ware

Szczepan W. Baran

Cell Surface Markers in Human Embryonic Stem Cells 51(12)

Raj R. Rao

Alison Venable Johnson

Steven L. Stice

Generation of a Monoclonal Antibody Library Against Human Embryonic Stem Cells 63(20)

Micha Drukker

Christina Muscat

Irving L. Weissman

Analytical Methods for Cancer Stem Cells 83(14)

Vinagolu K. Rajasekhar

Micro RNA Profiling: An Easy and Rapid Method to Screen and Characterize Stem Cell Populations 97(18)

Uma Lakshmipathy

Brad Love

Christopher Adams

Bhaskar Thyagarajan

Jonathan D. Chesnut

Gene Transfer Via Nucleofection Into Adult and Embryonic Stem Cells 115(12)

Uma Lakshmipathy

Shannon Buckley

Catherine Verfaillie

RNAi Knockdown of Transcription Factor Pu.1 in the Differentiation of Mouse Embryonic Stem Cells 127(10)

Gang-Ming Zou

Meredith A. Thompson

Mervin C. Yoder

StemBase: A Resource for the Analysis of Stem Cell Gene Expression Data 137(12)

Christopher J. Porter

Gareth A. Palidwor

Reatha Sandie

Paul M. Krzyzanowski

Enrique M. Muro

Carolina Perez-Iratxeta

Miguel A. Andrade-Navarro

Isolation of Stem Cells from Human Umbilical Cord Blood 149(16)

Nishanth P. Reddy

Mohan C. Vemuri

Reddanna Pallu

Ex Vivo Expansion of Hematopoietic Stem Cells from Human Cord Blood in Serum-Free Conditions 165(12)

Chao-Ling Yao

Shiaw-Min Hwang

Hematopoietic Colony-Forming Cell Assays 177(32)

Carla Pereira

Emer Clarke

Jackie Damen

Assays for Alloreactive Responses by PCR 209(16)

Patrick Stordeur

Immune Properties of Mesenchymal Stem Cells 225(20)

Panagiota A. Sotiropoulou

Michael Papamichail

Clinical Grade Expansion of Human Bone Marrow Mesenchymal Stem Cells 245(20)

Panagiota A. Sotiropoulou

Sonia A. Perez

Michael Papamichail

Adenoviral Transduction of Mesenchymal Stem Cells 265(10)

Pablo Bosch

Steven L. Stice

Directed Differentiation of Human Embryonic Stem Cells to Dendritic Cells 275(20)

Maxim A. Vodyanik

Igor I. Slukvin

Insulin-Producing Cells from Embryonic Stem Cells Experimental Considerations 295(16)

Enrique Roche

Roberto Ensenat-Waser

Nestor Vicente-Salar

Alfredo Santana

Martin Zenke

Juan Antonio Reig

Efficient Generation of Dopamine Neurons from Human Embryonic Stem Cells 311(12)

Chang-Hwan Park

Sang-Hun Lee

Isolation of Oligodendroglial Cells from Cultured Neural Stem/Progenitors 323(10)

Frank Zeigler

Stephen G. Hall

Differentiation of Human Embryonic Stem Cells Toward the Chondrogenic Lineage 333(18)

Wei Seong Toh

Zheng Yang

Boon Chin Heng

Tong Cao

Cartilage Tissue Engineering: Directed Differentiation of Embryonic Stem Cells in Three-Dimensional Hydrogel Culture 351(24)

Nathaniel S. Hwang

Shyni Varghese

Jennifer Elisseeff
Index 375

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