简介
Summary:
Publisher Summary 1
Because stem cells can grow and divide indefinitely and can also differentiate into specialized cell types, they promise significant advances in regenerative medicine. Recent work has led to the development of new methods for embryonic and adult stem cell isolation, characterization, expansion in undifferentiated state, customized differentiation, efficient stem cell transduction for gene therapy, quick monitoring of rejection, and other advances. This volume, edited by Vemuri (Invitrogen Corporation), presents a sample of methodological advances for wider use by novice and expert scientists, including derivation of human embryonic stem cells in xeno-free conditions, digital imaging of stem cells by electron microscopy, controlled-cooling protocol for cryopreservation of human and non-human primate embryonic stem cells, cell surface markers in human embryonic stem cells, generation of a monoclonal antibody library against human embryonic stem cells, gene transfer via nucleofection into adult and embryonic stem cells, isolation of stem cells from human umbilical cord blood in serum-free conditions, hematopoietic colony-forming cell assays, assays for alloreactive responses by polymerase chain reaction, adenoviral transduction of mesechymal stem cells, and 13 others. As in the rest of the series, each chapter describes laboratory protocols, necessary materials, troubleshooting, and known pitfalls. Annotation 漏2008 Book News, Inc., Portland, OR (booknews.com)
Publisher Summary 2
The scope for improving health care using stem cell therapies is thrilling, but has considerable technical challenges and methodological constraints that need to be addressed. Keeping with the tradition of Humana Press to bring these developments to the forefront in a timely manner, this book presents scientific advances in stem cell methods for a wider use by novice and expert scientists, through the series of Methods in Molecular Biology.
目录
Table Of Contents:
Preface v
Contributors xi
Derivation of Human Embryonic Stem Cells in Xeno-Free Conditions 1(10)
Mohan C. Vemuri
Tim Schimmel
Pere Colls
Santiago Munne
Jacques Cohen
Feeder-Layer Free Culture System for Human Embryonic Stem Cells 11(10)
Michal Amit
Digital Imaging of Stem Cells by Electron Microscopy 21(22)
A. Henry Sathananthan
Stefania A. Nottola
A Controlled-Cooling Protocol for Cryopreservation of Human and Non-Human Primate Embryonic Stem Cells 43(8)
Carol B. Ware
Szczepan W. Baran
Cell Surface Markers in Human Embryonic Stem Cells 51(12)
Raj R. Rao
Alison Venable Johnson
Steven L. Stice
Generation of a Monoclonal Antibody Library Against Human Embryonic Stem Cells 63(20)
Micha Drukker
Christina Muscat
Irving L. Weissman
Analytical Methods for Cancer Stem Cells 83(14)
Vinagolu K. Rajasekhar
Micro RNA Profiling: An Easy and Rapid Method to Screen and Characterize Stem Cell Populations 97(18)
Uma Lakshmipathy
Brad Love
Christopher Adams
Bhaskar Thyagarajan
Jonathan D. Chesnut
Gene Transfer Via Nucleofection Into Adult and Embryonic Stem Cells 115(12)
Uma Lakshmipathy
Shannon Buckley
Catherine Verfaillie
RNAi Knockdown of Transcription Factor Pu.1 in the Differentiation of Mouse Embryonic Stem Cells 127(10)
Gang-Ming Zou
Meredith A. Thompson
Mervin C. Yoder
StemBase: A Resource for the Analysis of Stem Cell Gene Expression Data 137(12)
Christopher J. Porter
Gareth A. Palidwor
Reatha Sandie
Paul M. Krzyzanowski
Enrique M. Muro
Carolina Perez-Iratxeta
Miguel A. Andrade-Navarro
Isolation of Stem Cells from Human Umbilical Cord Blood 149(16)
Nishanth P. Reddy
Mohan C. Vemuri
Reddanna Pallu
Ex Vivo Expansion of Hematopoietic Stem Cells from Human Cord Blood in Serum-Free Conditions 165(12)
Chao-Ling Yao
Shiaw-Min Hwang
Hematopoietic Colony-Forming Cell Assays 177(32)
Carla Pereira
Emer Clarke
Jackie Damen
Assays for Alloreactive Responses by PCR 209(16)
Patrick Stordeur
Immune Properties of Mesenchymal Stem Cells 225(20)
Panagiota A. Sotiropoulou
Michael Papamichail
Clinical Grade Expansion of Human Bone Marrow Mesenchymal Stem Cells 245(20)
Panagiota A. Sotiropoulou
Sonia A. Perez
Michael Papamichail
Adenoviral Transduction of Mesenchymal Stem Cells 265(10)
Pablo Bosch
Steven L. Stice
Directed Differentiation of Human Embryonic Stem Cells to Dendritic Cells 275(20)
Maxim A. Vodyanik
Igor I. Slukvin
Insulin-Producing Cells from Embryonic Stem Cells Experimental Considerations 295(16)
Enrique Roche
Roberto Ensenat-Waser
Nestor Vicente-Salar
Alfredo Santana
Martin Zenke
Juan Antonio Reig
Efficient Generation of Dopamine Neurons from Human Embryonic Stem Cells 311(12)
Chang-Hwan Park
Sang-Hun Lee
Isolation of Oligodendroglial Cells from Cultured Neural Stem/Progenitors 323(10)
Frank Zeigler
Stephen G. Hall
Differentiation of Human Embryonic Stem Cells Toward the Chondrogenic Lineage 333(18)
Wei Seong Toh
Zheng Yang
Boon Chin Heng
Tong Cao
Cartilage Tissue Engineering: Directed Differentiation of Embryonic Stem Cells in Three-Dimensional Hydrogel Culture 351(24)
Nathaniel S. Hwang
Shyni Varghese
Jennifer Elisseeff
Index 375
Preface v
Contributors xi
Derivation of Human Embryonic Stem Cells in Xeno-Free Conditions 1(10)
Mohan C. Vemuri
Tim Schimmel
Pere Colls
Santiago Munne
Jacques Cohen
Feeder-Layer Free Culture System for Human Embryonic Stem Cells 11(10)
Michal Amit
Digital Imaging of Stem Cells by Electron Microscopy 21(22)
A. Henry Sathananthan
Stefania A. Nottola
A Controlled-Cooling Protocol for Cryopreservation of Human and Non-Human Primate Embryonic Stem Cells 43(8)
Carol B. Ware
Szczepan W. Baran
Cell Surface Markers in Human Embryonic Stem Cells 51(12)
Raj R. Rao
Alison Venable Johnson
Steven L. Stice
Generation of a Monoclonal Antibody Library Against Human Embryonic Stem Cells 63(20)
Micha Drukker
Christina Muscat
Irving L. Weissman
Analytical Methods for Cancer Stem Cells 83(14)
Vinagolu K. Rajasekhar
Micro RNA Profiling: An Easy and Rapid Method to Screen and Characterize Stem Cell Populations 97(18)
Uma Lakshmipathy
Brad Love
Christopher Adams
Bhaskar Thyagarajan
Jonathan D. Chesnut
Gene Transfer Via Nucleofection Into Adult and Embryonic Stem Cells 115(12)
Uma Lakshmipathy
Shannon Buckley
Catherine Verfaillie
RNAi Knockdown of Transcription Factor Pu.1 in the Differentiation of Mouse Embryonic Stem Cells 127(10)
Gang-Ming Zou
Meredith A. Thompson
Mervin C. Yoder
StemBase: A Resource for the Analysis of Stem Cell Gene Expression Data 137(12)
Christopher J. Porter
Gareth A. Palidwor
Reatha Sandie
Paul M. Krzyzanowski
Enrique M. Muro
Carolina Perez-Iratxeta
Miguel A. Andrade-Navarro
Isolation of Stem Cells from Human Umbilical Cord Blood 149(16)
Nishanth P. Reddy
Mohan C. Vemuri
Reddanna Pallu
Ex Vivo Expansion of Hematopoietic Stem Cells from Human Cord Blood in Serum-Free Conditions 165(12)
Chao-Ling Yao
Shiaw-Min Hwang
Hematopoietic Colony-Forming Cell Assays 177(32)
Carla Pereira
Emer Clarke
Jackie Damen
Assays for Alloreactive Responses by PCR 209(16)
Patrick Stordeur
Immune Properties of Mesenchymal Stem Cells 225(20)
Panagiota A. Sotiropoulou
Michael Papamichail
Clinical Grade Expansion of Human Bone Marrow Mesenchymal Stem Cells 245(20)
Panagiota A. Sotiropoulou
Sonia A. Perez
Michael Papamichail
Adenoviral Transduction of Mesenchymal Stem Cells 265(10)
Pablo Bosch
Steven L. Stice
Directed Differentiation of Human Embryonic Stem Cells to Dendritic Cells 275(20)
Maxim A. Vodyanik
Igor I. Slukvin
Insulin-Producing Cells from Embryonic Stem Cells Experimental Considerations 295(16)
Enrique Roche
Roberto Ensenat-Waser
Nestor Vicente-Salar
Alfredo Santana
Martin Zenke
Juan Antonio Reig
Efficient Generation of Dopamine Neurons from Human Embryonic Stem Cells 311(12)
Chang-Hwan Park
Sang-Hun Lee
Isolation of Oligodendroglial Cells from Cultured Neural Stem/Progenitors 323(10)
Frank Zeigler
Stephen G. Hall
Differentiation of Human Embryonic Stem Cells Toward the Chondrogenic Lineage 333(18)
Wei Seong Toh
Zheng Yang
Boon Chin Heng
Tong Cao
Cartilage Tissue Engineering: Directed Differentiation of Embryonic Stem Cells in Three-Dimensional Hydrogel Culture 351(24)
Nathaniel S. Hwang
Shyni Varghese
Jennifer Elisseeff
Index 375
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