Patch-clamp analysis : advanced techniques /
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作 者：edited by Wolfgang Walz, Alan A. Boulton and Glen B. Baker.
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ISBN：9781588290137
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简介
Summary:
Publisher Summary 1
Twelve papers by academic researchers describe all the major applications involving patch pipets, including recent innovations, applications to molecular biology and imaging, cytoplasm manipulation, drug delivery, and the measurement of intracellular signals. The coverage for each technique explains its theoretical background, start-up instructions, step-by-step protocols, and advice for solving common problems and analyzing data. Numerous b&w images accompany the text. Annotation c. Book News, Inc., Portland, OR (booknews.com)
目录
Preface to the Series p. v
Preface p. vii
Contributors p. xi
1. Technology of Patch-Clamp Electrodes Richard A. Levis and James L. Rae p. 1
2. Whole-Cell Patch-Clamp Recordings Harald Sontheimer and Christopher B. Ransom p. 35
3. Single-Channel Recording David J. A. Wyllie p. 69
4. Combined Fluorometric and Electrophysiological Recordings Hartmut Schmidt and Jens Eilers p. 111
5. Patch-Clamp Techniques Applied to Brain Slices James R. Moyer, Jr. and Thomas H. Brown p. 135
6. Perforated Patch-Clamp Technique Raimondo D'Ambrosio p. 195
7. Fast-Drug Application Manfred Heckmann and Christian Pawlu p. 217
8. Pipet Perfusion Methods: Techniques and Applications Gea-Ny Tseng and Minoru Horie p. 231
9. Patch-Cram Detection of Cyclic GMP in Intact Cells Richard H. Kramer p. 245
10. Loose-Patch Voltage-Clamp Technique Blake D. Anson and William M. Roberts p. 265
11. Recording from Macropatches Frances Moody-Corbett and Mark Fry p. 287
12. Combining Patch-Clamp Techniques with RT-PCR Gerald Seifert and Albert Becker and Christian Steinhauser p. 301
Index p. 331
Preface p. vii
Contributors p. xi
1. Technology of Patch-Clamp Electrodes Richard A. Levis and James L. Rae p. 1
2. Whole-Cell Patch-Clamp Recordings Harald Sontheimer and Christopher B. Ransom p. 35
3. Single-Channel Recording David J. A. Wyllie p. 69
4. Combined Fluorometric and Electrophysiological Recordings Hartmut Schmidt and Jens Eilers p. 111
5. Patch-Clamp Techniques Applied to Brain Slices James R. Moyer, Jr. and Thomas H. Brown p. 135
6. Perforated Patch-Clamp Technique Raimondo D'Ambrosio p. 195
7. Fast-Drug Application Manfred Heckmann and Christian Pawlu p. 217
8. Pipet Perfusion Methods: Techniques and Applications Gea-Ny Tseng and Minoru Horie p. 231
9. Patch-Cram Detection of Cyclic GMP in Intact Cells Richard H. Kramer p. 245
10. Loose-Patch Voltage-Clamp Technique Blake D. Anson and William M. Roberts p. 265
11. Recording from Macropatches Frances Moody-Corbett and Mark Fry p. 287
12. Combining Patch-Clamp Techniques with RT-PCR Gerald Seifert and Albert Becker and Christian Steinhauser p. 301
Index p. 331
Patch-clamp analysis : advanced techniques /
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