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简介
Surveys current knowledge of biological transmembrane molecular transport systems, and the investigational approaches used to study them. The 16 contributions characterize transporter families, describe genetic approaches to understanding transmembrane transporters, and examine functional aspects of transporter biology. Topics include yeast as a model system for studying glucose transport, peptide mapping of dopamine transporter ligand and substrate interaction sites, mass spectroscopy of membrane transport proteins, and voltage clamp and fluorometric techniques for studying glutamate transporter function. Annotation c. Book News, Inc., Portland, OR (booknews.com)
目录
Series Preface p. vii
Preface p. ix
Contributors p. xi
1 Families of Transporters and Their Classification Milton H. Saier, Jr. p. 1
2 Yeast as a Model System for Studying Glucose Transport Eckhard Boles p. 19
3 Neurotransmitter Transporters of Drosophila Martin G. Burg and William L. Pak p. 37
4 Transgenic Mice in Monoamine Transporter Research Sara R. Jones p. 51
5 Searching for Novel Genetic Variation in Neurotransmitter Transporters Randy D. Blakely and Alexandra R. Belous p. 65
6 Nonviral Gene Transfer Allows Up- and Down-Expression of the Brain Serotonin Transporter with Functional Consequences Marie-Pascale Martres and Veronique Fabre and Michel Hamon and Barbara Demeneix p. 89
7 Methods in Studying the Regulation and Trafficking of Transmembrane Transporters Scott L. Deken and Dan Wang and Michael W. Quick p. 111
8 Chemical Modification Strategies for Structure-Function Studies Gary Rudnick p. 125
9 Methanethiosulfonate Reagent Accessibility Studies, Cysteine-Scanning Mutagenesis, Protein Overexpression, and Functional Reconstitution: A Strategy for Studying the Structure/Function Relationships within the Mitochondrial Citrate Transport Protein Ronald S. Kaplan p. 143
10 Peptide Mapping of Dopamine Transporter Ligand and Substrate Interaction Sites Margaret J. Lowe and Jon D. Gaffaney and Roxanne A. Vaughan p. 161
11 Mass Spectrometry of Membrane Transport Proteins Julian P. Whitelegge and H. Ronald Kaback and Johannes le Coutre p. 179
12 Amperometric Recording of Amphetamine-Induced Dopamine Efflux David Sulzer and Kristopher M. Kahlig and Yvonne Schmitz and Christine Saunders and Aurelio Galli p. 191
13 Voltage Clamp and Fluorometric Techniques for Studying Glutamate Transporter Function Anastassios V. Tzingounis and H. Peter Larsson and Michael P. Kavanaugh p. 203
14 Studies of Glial Glutamate Transporters in Hippocampal Microcultures Steven Mennerick and Robert J. Cormier and Charles F. Zorumski p. 217
15 Imaging Monoamine Transporters in the Brain Hank F. Kung and Mei-Ping Kung p. 239
16 Measuring and Modeling the Spatiotemporal Profile of GABA at the Synapse Linda S. Overstreet and Gary L. Westbrook and Mathew V. Jones p. 259
Index p. 277
Preface p. ix
Contributors p. xi
1 Families of Transporters and Their Classification Milton H. Saier, Jr. p. 1
2 Yeast as a Model System for Studying Glucose Transport Eckhard Boles p. 19
3 Neurotransmitter Transporters of Drosophila Martin G. Burg and William L. Pak p. 37
4 Transgenic Mice in Monoamine Transporter Research Sara R. Jones p. 51
5 Searching for Novel Genetic Variation in Neurotransmitter Transporters Randy D. Blakely and Alexandra R. Belous p. 65
6 Nonviral Gene Transfer Allows Up- and Down-Expression of the Brain Serotonin Transporter with Functional Consequences Marie-Pascale Martres and Veronique Fabre and Michel Hamon and Barbara Demeneix p. 89
7 Methods in Studying the Regulation and Trafficking of Transmembrane Transporters Scott L. Deken and Dan Wang and Michael W. Quick p. 111
8 Chemical Modification Strategies for Structure-Function Studies Gary Rudnick p. 125
9 Methanethiosulfonate Reagent Accessibility Studies, Cysteine-Scanning Mutagenesis, Protein Overexpression, and Functional Reconstitution: A Strategy for Studying the Structure/Function Relationships within the Mitochondrial Citrate Transport Protein Ronald S. Kaplan p. 143
10 Peptide Mapping of Dopamine Transporter Ligand and Substrate Interaction Sites Margaret J. Lowe and Jon D. Gaffaney and Roxanne A. Vaughan p. 161
11 Mass Spectrometry of Membrane Transport Proteins Julian P. Whitelegge and H. Ronald Kaback and Johannes le Coutre p. 179
12 Amperometric Recording of Amphetamine-Induced Dopamine Efflux David Sulzer and Kristopher M. Kahlig and Yvonne Schmitz and Christine Saunders and Aurelio Galli p. 191
13 Voltage Clamp and Fluorometric Techniques for Studying Glutamate Transporter Function Anastassios V. Tzingounis and H. Peter Larsson and Michael P. Kavanaugh p. 203
14 Studies of Glial Glutamate Transporters in Hippocampal Microcultures Steven Mennerick and Robert J. Cormier and Charles F. Zorumski p. 217
15 Imaging Monoamine Transporters in the Brain Hank F. Kung and Mei-Ping Kung p. 239
16 Measuring and Modeling the Spatiotemporal Profile of GABA at the Synapse Linda S. Overstreet and Gary L. Westbrook and Mathew V. Jones p. 259
Index p. 277
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