Medical functional experimentation

《机能实验学（第二版）（英文版）》

chapter 1. an introduction of functional experimentation (1)

chapter 2. basic knowledge and theory(7)

section 1. biological characteristics and application of commonly used laboratory animals (7)

section 2. biological data acquisition systems (13)

section 3. selection and design principles of medical scientific research topics (20)

section 4. medical information retrieval (27)

section 5. writing methods of scientific papers and lab reports (30)

section 6. laboratory safety and rules (35)

chapter 3. basic methods of animal experiments (43)

section 1. animal capture and restraint (43)

section 2. animal numbering (46)

section 3. drug administration for laboratory animals (48)

section 4. anesthesia of laboratory animals (52)

section 5. unhairing of laboratory animals (57)

section 6. blood collection of laboratory animals (58)

section 7. euthanasia methods of laboratory animals (65)

chapter 4. basic techniques in experimental animal surgical operation (67)

section 1. traditional surgical instruments (67)

section 2. technique of animal catheterization (69)

.section 3. haemostasis and technique of knots making (72)

section 4. technique of wound sutures (stitches)and sutures out (74)

chapter 5. basic experiments (76)

experiment 1. the nature of muscle contraction (76)

experiment 2. measurement of conduction velocity of action potential and excitability from neural trunk (78)

experiment 3. determination of hemoglobin content and red blood cell osmotic fragihty (82)

experiment 4. premature contraction and compensatory pause (84)

experiment 5. chemicals on the regulation of ex vivo heart activities (86)

experiment 6. measurement of human arterial blood pressure (88)

experiment 7. recording of human electrocardiogram (91)

experiment 8. pulmonary function tests (93)

experiment 9. regulation of respiratory movements (96)

experiment 10. physiological properties of the isolated gastrointestinal smooth muscle (98)

experiment 11. factors that influencing urine formation (100)

experiment 12. damage effects of unilateral labyrinth and cochlear potential induction from guinea pigs (103)

experiment 13. examination of visual fields and scotornas (105)

experiment 14. analysis of reflex arc and the basic features of the reflex activities (107)

experiment 15. experimental pulmonary edema in rabbits (111)

experiment 16. effect of different factors on hypoxic tolerance (113)

experiment 17. influence of ph of body fluids on drug absorption (114)

experiment 18. one-compartment open model and calculation of the pharmacokinetic parameters (115)

experiment 19. two-compartment open model and calculation of the pharmacokinetic parameters (119)

experiment 20. determination of medial lethal dose (lds0) of drug (122)

experiment 21. the antergic experiments of drng-the binding assay of radio-ligand and receptor (123)

experiment 22. intoxication and detoxication of organophosphates (126)

experiment 23. effects of drugs by different dosage forms (128)

experiment 24. effects of drugs by different routes of administration (130)

experiment 25. basic effects of drugs (131)

experiment 26. factors influencing drug effects (133)

experiment 27. observation of analgesic action of drugs with writhing method (136)

experiment 28. anticonvulsant effect of the drug (137)

experiment 29. effects of drugs on learning and memory of animal (mice step-down experiment. rat shuttle box experiment. morris water maze experiment) (139)

experiment 30. effects of hydrocortisone on mice ear swelling induced by xylol (144)

experiment 31. anti-inflammatory effects of dexamethasone on rat paw edema (145)

experiment 32. effects of cardiac glycosides on heart failure (147)

experiment 33. antibacterial experiment of antibacterial agents in vitro (150)

experiment 34. antibacterial experiment of antibacterial agents in vivo (152)

chapter 6. comprehensive experiments (157 )

experiment 1. different types of hypoxia (157)

experiment 2. hyperkalemia in rabbits (159)

experiment 3. disseminated intravascular coagulation in rabbit (161)

experiment 4. disorders of acid-base balance and its experimental therapy in rabbits (166)

experiment 5. ischemia and reperfusion injury in intestine (170)

experiment 6. acute endotoxic shock (172)

experiment 7. hemorrhagic shock and alterations of microcirculation in rabbit (175)

experiment 8. regulation of the cardiovascular system and acute cardiac failure (178)

experiment 9. effects of experimental pneumothorax on respiration. circulation and acid-base balance in rabbits (183)

experiment 10. acute respiratory distress syndrome and pharmacotherapy (186)

experiment 11. hepatic encephalopathy and its treatment (189)

experiment 12. determination of endogenous creatinine clearance rate and excretion fraction of sodium filtration in acute renal failure (192)

experiment 13. reproduction of multiple organ dysfunction syndrome (193)

experiment 14. the effect of drug and bioactivator on the isolated tracheal strips of cavy (198)

experiment 15. determination of the pharmacokinetic parameters of sulfa drugs in rabbits with renal failure (200)

chapter 7. exploratory experiments (204)

chapter 8. case discussion and paper analysis (205)

references (239)

appendix (240)