Preface p. v
Contributors p. xi
1 Plasmid Vectors Nicola Casali and Sabine Ehrt p. 1
2 Isolation of DNA from Mycobacterium tuberculosis Paul D. van Helden and Thomas C. Victor and Robin M. Warren and Eileen G. van Helden p. 19
3 Extraction of RNA from Intracellular Mycobacterium tuberculosis: Methods, Considerations, and Applications Irene M. Monahan and Joseph A. Mangan and Philip D. Butcher p. 31
4 Transposon Mutagenesis in Mycobacteria Using Conditionally Replicating Mycobacteriophages Stoyan S. Bardarov and Svetoslav S. Bardarov, Jr. and William R. Jacobs, Jr. p. 43
5 Gene Replacement and Transposon Delivery Using the Negative Selection Marker sacB Mary Jackson and Luis Reinaldo Camacho and Brigitte Gicquel and Christophe Guilhot p. 59
6 Gene Replacement Using Pretreated DNA Bhavna G. Gordhan and Tanya Parish p. 77
7 Gene Replacement in Mycobacterium tuberculosis and Mycobacterium bovis BCG Using rpsL+ as a Dominant Negative Selectable Marker Peter Sander and Burkhard Springer and Erik C. Bottger p. 93
8 Transcription Start-Site Mapping Farahnaz Movahedzadeh and Jorge A. Gonzalez-Y-Merchand and Robert A. Cox p. 105
9 Fluorescence and Brightfield Cytology of Live M. tuberculosis Cells Rory P. Cooney and Natalie J. Garton and Michael R. Barer p. 125
10 Phage Replication Technology for Diagnosis and Drug Susceptibility Testing Ruth McNerney p. 145
11 Detection of Mutations in Mycobacterium tuberculosis by a Dot Blot Hybridization Strategy Thomas C. Victor and Paul D. van Helden p. 153
12 Restriction Fragment Length Polymorphism Typing of Mycobacteria Dick van Soolingen and Petra E. W. de Haas and Kristin Kremer p. 165
13 Preparation of Culture Filtrate Proteins from Mycobacterium tuberculosis Ida Rosenkrands and Peter Andersen p. 205
14 Analysis of the Capsule of Mycobacterium tuberculosis Mamadou Daffe and Marie-Antoinette Laneelle p. 217
15 Analysis of the Lipids of Mycobacterium tuberculosis Richard A. Slayden and Clifton E. Barry p. 229
16 In Vitro Model of Hypoxically Induced Nonreplicating Persistence of Mycobacterium tuberculosis Lawrence G. Wayne p. 247
17 Macrophage Virulence Assays Pauline T. Lukey and Elizabeth U. Hooker p. 271
18 Analysis of Mycobacterium-Infected Macrophages by Immunoelectron Microscopy and Cell Fractionation Wandy Beatty and David G. Russell p. 281
19 Real-Time PCR Using Molecular Beacons: A New Tool to Identify Point Mutations and to Analyze Gene Expression in Mycobacterium tuberculosis Riccardo Manganelli and Sanjay Tyagi and Issar Smith p. 295
20 Electronic Access to Mycobacterium tuberculosis Sequence Data Julian Parkhill p. 311
21 Proteomics Joanna C. Betts and Marjorie A. Smith p. 315
22 Functional Genomics of Mycobacterium tuberculosis Using DNA Microarrays Michael Wilson and Martin Voskuil and Dirk Schnappinger and Gary K. Schoolnik p. 335
23 Storage of Mycobacterial Strains Kristin Kremer and Tridia van der Laan and Dick van Soolingen p. 359
24 Safety in the Laboratory Heidi Alderton and Debbie Smith p. 367
Index p. 385