Proteomics sample preparation /

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作   者:edited by J?rg von Hagen.

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ISBN:9783527317967

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简介

This long-awaited first guide to sample preparation for proteomics studies overcomes a major bottleneck in this fast growing technique within the molecular life sciences. By addressing the topic from three different angles -- sample, method and aim of the study -- this practical reference has something for every proteomics researcher. Following an introduction to the field, the book looks at sample preparation for specific techniques and applications and finishes with a section on the preparation of sample types. For each method described, a summary of the pros and cons is given, as well as step-by-step protocols adaptable to any specific proteome analysis task.

目录

Perspectives In Proteomics Sample Preparation
Introduction General Aspects of Sample Preparation for Comprehensive
Proteome Analysis Proteomics: A Philosophical Perspective MASS SPECTROMETRY
A Practical Guideline to Electrospray Ionization Mass Spectrometry for Proteomics Application
Sample Preparation for the Application of MALDI Mass Spectrometry
Sample Preparation for Label-Free Proteomic Analyses of Body Fluids by Fourier
Transform Ion Cyclotron Mass Spectrometry Sample Preparation for Differential Proteome Analysis: Labelling Technologies for Mass
Spectrometr Determining Protein Localization within Subcellular Compartments using Stable IsotopeTagging
Electrophoresis Sample Preparation for Two-Dimensional Gel Electrophoresis
Sample Preparation for Native Electrophoresis
Sample Preparation for LC-MS/MS using Free Flow Electrophoresis
Sample Preparation for Proteomics
Optical Methods High-Throughout Proteomics: Spinning Disc Interferometry (SDI)
Optical Proteomics on Cell Arrays Sample Preparation by Laser Microdissection and Catapulting for Proteome
Analysis Sample Preparation for Flow Cytometry
Chromatography Sample Preparation for HPLC-Based Proteome
Analysis Sample Preparation for Two-Dimensional Phosphopeptide
Mapping and Phosphoamino Acid Analysis
Structural Proteomics Exploring Protein-Ligand Interactions by Solution NMR
Sample Preparation for Crystallography INTERACTION ANALYSIS
Sample Preparation for Protein Complex Analysis by the Tandem Affinity Purification (TAP)
Methods Exploring Membrane Proteomes Post-Translational Modifications Sample Preparation for Phosphoproteome
Analysis Sample Preparation for Analysis of Post-Translational Modifications: Glycosylation
Species-Dependent Proteomics Sample Preparation and Data Processing in Plant Proteomics Sample Preparation for MudPIT with Bacterial
Protein Samples Sample Preparation for the Cell-Wall Proteome Analysis of Yeast and Fungi
The Human Proteosome Clinical Proteomics: Sample Preparation and Standardization Stem Cell Proteomics BIOINFORMATICS
Bioinformatics Support for Mass Spectrometric Quality Control Use of Physio-Chemical Properties in Peptide and Protein Identification

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    东野圭吾 (作者), 李盈春 (译者)

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