Handbook of plant ecophysiology techniques /
副标题:无
作 者:edited by Manuel J. Reigosa Roger.
分类号:
ISBN:9780792370536
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简介
Summary:
Publisher Summary 1
This book is intended for people interested in plant ecophysiology research or related sciences. It will also be useful for scientists working in forestry or agronomy. Twenty-seven chapters cover a wide variety of techniques, from electron microscopy applied to seed germination, flow cytometry applied to different measures, cell cycle studies, field and lab measures related to photosynthesis (from pigments to gas exchange and fluorescence), determination of water-related parameters, and techniques to measure phenolics, total soluble proteins, stress proteins, polyamines, proline, RubisCO activity, ATPases, ABA, and ion interchange. Each chapter includes both physiological aspects of the measured parameter and the chosen reference technique for that parameter. A special emphasis is put on allelopathy research, although the book will be valuable for the study of any kind of stress. All chapters can be read independently, and both expert and inexperienced scientists will be able to take advantage of the handbook.
目录
Table Of Contents:
List of Contributors ix
Preface xi
Acknowledgments xv
Seed Bioassay and microscopy in the study of allelopathy: radish and purslane responses 1(20)
Giovanni Aliotta
Gennaro Cafiero
Flow cytometry: principles and Instrumentation 21(14)
Alfonso Blanco Fernandez
Adela M. Sanchez-Moreiras
Teodoro Coba de la Pena
Flow cytometry: DNA quantification 35(18)
Alfonso Blanco Fernandez
Use of flow cytometry to measure physiological parameters 53(12)
Teodoro Coba de la Pena
Flow cytometry: cell cycle 65(16)
Teodoro Coba de la Pena
Adela Sanchez-Moreiras
Mitotic index 81(16)
Adela M. Sanchez-Moreiras
Teodoro Coba de la Pena
Ana Martinez Otero
Alfonso Blanco Fernandez
Determination of photosynthetic pigments 97(16)
Luis Gonzalez
Gas exchange techniques in photosynthesis and respiration. Infrared Gas Analyzer 113(28)
Pilar Ramos Tamayo
Oliver Weiss
Adela Sanchez Moreiras
Use of oxygen electrode in measurements of photosynthesis and respiration 141(14)
Luis Gonzalez
Carlos Bolano
Francois Pellissier
Fluorescence techniques 155(18)
Manuel J. Reigosa Roger
Oliver Weiss
Modulated fluorescence 173(12)
Oliver Weiss
Manuel J. Reigosa Roger
Plant water status 185(8)
Luis Gonzalez
Manuel J. Reigosa Roger
Determination of water potential in leaves 193(14)
Luis Gonzalez
Determination of relative water content 207(6)
Luis Gonzalez
Marco Gonzalez-Vilar
Determination of water potential in soils 213(10)
Luis Gonzalez
Determination of transpiration using a steady-state porometer 223(12)
Manuel J. Reigosa Roger
Adela Sanchez-Moreiras
Stomata imprints. A new and quick method to count stomata and epidermis cells 235(16)
Margit H. Meister
Harald R. Bolhar-Nordenkampf
HPLC Techniques - Phenolics 251(32)
X. Carlos Souto
J. Carlos Bolano
Luis Gonzalez
Xoan X. Santos
Protein content quantification by Bradford method 283(14)
Nuria Pedrol Bonjoch
Pilar Ramos Tamayo
Two-dimensional electrophoresis. Stress proteins 297(38)
Adela M. Sanchez-Moreiras
Nuria Pedrol Bonjoch
Polyamines determination by TLC and HPLC 335(30)
Nuria Pedrol
Antonio F. Tiburcio
Free proline quantification 365(18)
Pilar Ramos Tamayo
Nuria Pedrol Bonjoch
Assessment of D-Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) enzymatic activity 383(16)
Teodoro Coba de la Pena
Adela Sanchez Moreiras
Xoan Xose Santos Costa
Ana Martinez Otero
ATP phosphohydrolase activity 399(14)
Adela M. Sanchez-Moreiras
Root uptake and release of ions 413(16)
Adela M. Sanchez-Moreiras
Oliver Weiss
Radioinmunoassay of Abscisic Acid 429(14)
Mark A. Bacon
Radiochemical techniques to quantify allelochemical in plants 443
Genevieve Chiapusio
Francois Pellissier
List of Contributors ix
Preface xi
Acknowledgments xv
Seed Bioassay and microscopy in the study of allelopathy: radish and purslane responses 1(20)
Giovanni Aliotta
Gennaro Cafiero
Flow cytometry: principles and Instrumentation 21(14)
Alfonso Blanco Fernandez
Adela M. Sanchez-Moreiras
Teodoro Coba de la Pena
Flow cytometry: DNA quantification 35(18)
Alfonso Blanco Fernandez
Use of flow cytometry to measure physiological parameters 53(12)
Teodoro Coba de la Pena
Flow cytometry: cell cycle 65(16)
Teodoro Coba de la Pena
Adela Sanchez-Moreiras
Mitotic index 81(16)
Adela M. Sanchez-Moreiras
Teodoro Coba de la Pena
Ana Martinez Otero
Alfonso Blanco Fernandez
Determination of photosynthetic pigments 97(16)
Luis Gonzalez
Gas exchange techniques in photosynthesis and respiration. Infrared Gas Analyzer 113(28)
Pilar Ramos Tamayo
Oliver Weiss
Adela Sanchez Moreiras
Use of oxygen electrode in measurements of photosynthesis and respiration 141(14)
Luis Gonzalez
Carlos Bolano
Francois Pellissier
Fluorescence techniques 155(18)
Manuel J. Reigosa Roger
Oliver Weiss
Modulated fluorescence 173(12)
Oliver Weiss
Manuel J. Reigosa Roger
Plant water status 185(8)
Luis Gonzalez
Manuel J. Reigosa Roger
Determination of water potential in leaves 193(14)
Luis Gonzalez
Determination of relative water content 207(6)
Luis Gonzalez
Marco Gonzalez-Vilar
Determination of water potential in soils 213(10)
Luis Gonzalez
Determination of transpiration using a steady-state porometer 223(12)
Manuel J. Reigosa Roger
Adela Sanchez-Moreiras
Stomata imprints. A new and quick method to count stomata and epidermis cells 235(16)
Margit H. Meister
Harald R. Bolhar-Nordenkampf
HPLC Techniques - Phenolics 251(32)
X. Carlos Souto
J. Carlos Bolano
Luis Gonzalez
Xoan X. Santos
Protein content quantification by Bradford method 283(14)
Nuria Pedrol Bonjoch
Pilar Ramos Tamayo
Two-dimensional electrophoresis. Stress proteins 297(38)
Adela M. Sanchez-Moreiras
Nuria Pedrol Bonjoch
Polyamines determination by TLC and HPLC 335(30)
Nuria Pedrol
Antonio F. Tiburcio
Free proline quantification 365(18)
Pilar Ramos Tamayo
Nuria Pedrol Bonjoch
Assessment of D-Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) enzymatic activity 383(16)
Teodoro Coba de la Pena
Adela Sanchez Moreiras
Xoan Xose Santos Costa
Ana Martinez Otero
ATP phosphohydrolase activity 399(14)
Adela M. Sanchez-Moreiras
Root uptake and release of ions 413(16)
Adela M. Sanchez-Moreiras
Oliver Weiss
Radioinmunoassay of Abscisic Acid 429(14)
Mark A. Bacon
Radiochemical techniques to quantify allelochemical in plants 443
Genevieve Chiapusio
Francois Pellissier
Handbook of plant ecophysiology techniques /
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