Handbook of plant ecophysiology techniques /

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作   者:edited by Manuel J. Reigosa Roger.

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ISBN:9780792370536

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简介

Summary: Publisher Summary 1 This book is intended for people interested in plant ecophysiology research or related sciences. It will also be useful for scientists working in forestry or agronomy. Twenty-seven chapters cover a wide variety of techniques, from electron microscopy applied to seed germination, flow cytometry applied to different measures, cell cycle studies, field and lab measures related to photosynthesis (from pigments to gas exchange and fluorescence), determination of water-related parameters, and techniques to measure phenolics, total soluble proteins, stress proteins, polyamines, proline, RubisCO activity, ATPases, ABA, and ion interchange. Each chapter includes both physiological aspects of the measured parameter and the chosen reference technique for that parameter. A special emphasis is put on allelopathy research, although the book will be valuable for the study of any kind of stress. All chapters can be read independently, and both expert and inexperienced scientists will be able to take advantage of the handbook.  

目录

Table Of Contents:

List of Contributors ix
Preface xi
Acknowledgments xv

Seed Bioassay and microscopy in the study of allelopathy: radish and purslane responses 1(20)

Giovanni Aliotta

Gennaro Cafiero

Flow cytometry: principles and Instrumentation 21(14)

Alfonso Blanco Fernandez

Adela M. Sanchez-Moreiras

Teodoro Coba de la Pena

Flow cytometry: DNA quantification 35(18)

Alfonso Blanco Fernandez

Use of flow cytometry to measure physiological parameters 53(12)

Teodoro Coba de la Pena

Flow cytometry: cell cycle 65(16)

Teodoro Coba de la Pena

Adela Sanchez-Moreiras

Mitotic index 81(16)

Adela M. Sanchez-Moreiras

Teodoro Coba de la Pena

Ana Martinez Otero

Alfonso Blanco Fernandez

Determination of photosynthetic pigments 97(16)

Luis Gonzalez

Gas exchange techniques in photosynthesis and respiration. Infrared Gas Analyzer 113(28)

Pilar Ramos Tamayo

Oliver Weiss

Adela Sanchez Moreiras

Use of oxygen electrode in measurements of photosynthesis and respiration 141(14)

Luis Gonzalez

Carlos Bolano

Francois Pellissier

Fluorescence techniques 155(18)

Manuel J. Reigosa Roger

Oliver Weiss

Modulated fluorescence 173(12)

Oliver Weiss

Manuel J. Reigosa Roger

Plant water status 185(8)

Luis Gonzalez

Manuel J. Reigosa Roger

Determination of water potential in leaves 193(14)

Luis Gonzalez

Determination of relative water content 207(6)

Luis Gonzalez

Marco Gonzalez-Vilar

Determination of water potential in soils 213(10)

Luis Gonzalez

Determination of transpiration using a steady-state porometer 223(12)

Manuel J. Reigosa Roger

Adela Sanchez-Moreiras

Stomata imprints. A new and quick method to count stomata and epidermis cells 235(16)

Margit H. Meister

Harald R. Bolhar-Nordenkampf

HPLC Techniques - Phenolics 251(32)

X. Carlos Souto

J. Carlos Bolano

Luis Gonzalez

Xoan X. Santos

Protein content quantification by Bradford method 283(14)

Nuria Pedrol Bonjoch

Pilar Ramos Tamayo

Two-dimensional electrophoresis. Stress proteins 297(38)

Adela M. Sanchez-Moreiras

Nuria Pedrol Bonjoch

Polyamines determination by TLC and HPLC 335(30)

Nuria Pedrol

Antonio F. Tiburcio

Free proline quantification 365(18)

Pilar Ramos Tamayo

Nuria Pedrol Bonjoch

Assessment of D-Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) enzymatic activity 383(16)

Teodoro Coba de la Pena

Adela Sanchez Moreiras

Xoan Xose Santos Costa

Ana Martinez Otero

ATP phosphohydrolase activity 399(14)

Adela M. Sanchez-Moreiras

Root uptake and release of ions 413(16)

Adela M. Sanchez-Moreiras

Oliver Weiss

Radioinmunoassay of Abscisic Acid 429(14)

Mark A. Bacon

Radiochemical techniques to quantify allelochemical in plants 443

Genevieve Chiapusio

Francois Pellissier

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