Non-conventional yeasts in genetics, biochemistry and, biotechnology : practical protocols /

Arxula adeninivorans
Chapter 1 Temperature-Dependent Dimorphism of the Non-Conventional Yeast Arxula adeninivorans T. Wartmann and G. Kunze p. 3
Chapter 2 Expression of Heterologous Genes in Arxula adeninivorans Budding Cells and Mycelia T. Wartmann and G. Kunze p. 7
Ashbya gossypii
Chapter 3 Effect of Precursor Feeding on Riboflavin Production of Ashbya gossypii and Influence of Threonine Aldolase Overexpression K-P. Stahmann and U. Weber p. 15
Candida maltosa
Chapter 4 Characterization of the 26S-rRNA Gene to Classify an Industrial Strain to be Candida maltosa M. Arie and H. Matsuda and M. Takagi p. 23
Chapter 5 Isolation of a GCN4 Gene Analog and Determination of Its Involvement in Induction of Cycloheximide-Resistant Ribosomal Protein LA1-Q in Candida maltosa H. Takaku and M. Takagi and A. Ohta p. 29
Chapter 6 Dehalogenation During Biodegradation of Chlorophenols by Candida maltosa E. Hammer p. 35
Chapter 7 Production of Aromatic Acids During the Oxidation of Phenylalkanes by Candida maltosa F. Schauer p. 39
Debaryomyces hansenii
Chapter 8 Growth of Debaryomyces hansenii in Seawater Culture Medium M. Ramirez-Orozco and JL. Ochoa p. 47
Chapter 9 Glycerol and Glucose Dissimilation by Debaryomyces hansenii: Substrate Influence on Growth Rate and Biomass Yield of Yeast JL. Ochoa and M. Ramirez-Orozco and F. Marquez p. 51
Chapter 10 Induction of GDP from Halotolerant Yeast Debaryomyces hansenii by Salt Stress PE. Thome p. 59
Chapter 11 Salt Tolerance in Debaryomyces hansenii. Effect of Sodium on Growth at High Temperature and at Extreme pH A. Almagro and J. Ramos p. 65
Chapter 12 Preparation of Total DNA from Debaryomyces hansenii A. Almagro and J. Ramos p. 69
Hansenula polymorpha
Chapter 13 Life Cycle of Hansenula polymorpha C. Gancedo and JM. Siverio and JM. Cregg p. 77
Chapter 14 Obtention of Auxotrophic Mutants in Hansenula polymorpha C. Gancedo and JM. Siverio and IM. Cregg p. 83
Chapter 15 Transformation by Electroporation of the Yeast Hansenula polymorpha C. Gancedo and JM. Siverio and JM. Cregg p. 89
Chapter 16 Expression of Hansenula polymorpha Nitrate Reductase Under the Control of the MOX1 Promoter C. Gancedo and JM. Siverio and JM. Cregg p. 97
Chapter 17 Promoter Assessment in H. polymorpha Using a lacZ Reporter Gene M. Suckow and M. Kutzner and C. Amuel and CP. Hollenberg and G. Gellissen p. 101
Chapter 18 Immuno-Gold Labeling of Invertase in Hansenula polymorpha I. Manazzu and R. Strabbioli p. 117
Hortaea werneckii
Chapter 19 Determination of Intra- and Extracellular Concentration of Glycerol in the Halophilic Black Yeast Hortaea werneckii Grown at Different Environmental Salinities U. Petrovic and A. Plemenitas p. 127
Chapter 20 Regulation of HMGCoA Reductase Activity by Salt Stress in Hortaea werneckii U. Petrovic and A. Plemenitas p. 131
Chapter 21 Isolation and Identification of Lipids from the Halophilic Black Yeast Hortaea werneckii M. Turk and L. Mejanelle and A. Plemenitas p. 135
Chapter 22 Inhibition of DHN-Melanin Biosynthesis by Tricyclazole in Hortaea werneckii T. Kogej and MH. Wheeler and T. Lanisnik Rizner and N. Gunde-Cimerman p. 143
Kluyveromyces lactis
Chapter 23 Mating, Sporulation, and Tetrad Analysis in Kluyveromyces lactis BJM. Zonneveld and HY. Steensma p. 151
Chapter 24 Isolation of Mitochondrial Mutants from the Petite-Negative Yeast Kluyveromyces lactis XJ. Chen p. 155
Chapter 25 Gene Disruption in the Yeast Kluyveromyces lactis O. Queiros and M. Casal and P. Moradas-Ferreira and C. Leao p. 161
Chapter 26 Transformation of Kluyveromyces lactis RA. Kooistra and HY. Steensma p. 169
Chapter 27 Removal of Dominant Markers from the Kluyveromyces lactis Genome Using the Cre/loxP System HY. Steensma p. 175
Chapter 28 Phenotypic Analysis of the Kluyveromyces lactis Killer Phenomenon L. Fichtner and D. Jablonowski and F. Frohloff and R. Schaffrath p. 179
Chapter 29 Genetic Manipulation of the Kluyveromyces lactis Killer Plasmids k1 and k2 R. Schaffrath p. 185
Chapter 30 Chitin-Binding Capacity of the Zymocin Complex from Kluyveromyces lactis D. Jablonowski and L. Fichtner and F. Frohloff and R. Schaffrath p. 191
Chapter 31 Heterologous Protein Production in High Copy Number Vector Systems MM. Bianchi and GB. Morlino and L. Frontali p. 185
Chapter 32 Alcohol Dehydrogenase (ADH) Isozymes in K. lactis: Detection by Activity C. Falcone and C. Mazzoni and M. Saliola p. 201
Chapter 33 Kluyveromyces lactis Genome Mapping and Sequencing M. Bolotin-Fukuhara p. 209
Pichia stipitis
Chapter 34 Mating and Segregation in Pichia stipitis B. Schruff and V. Passoth and U. Klinner p. 215
Chapter 35 ADH1 Disruption in P. stipitis by Targeted Restriction Enzyme Mediated Integration S. Fluthgraf and B. Schruff and U. Klinner p. 221
Chapter 36 Examination of Xylose Fermentation in P. stipitis by Means of the Sensomat System S. Fluthgraf and A. Kirchhoff and J. Debye and V. Passoth and U. Klinner p. 229
Chapter 37 Regulation of Fermentation and Respiration V. Passoth and B. Hahn-Hagerdal and U. Klinner p. 236
Chapter 38 Investigation of Transcriptional Regulation of the Fermentative ADH in Pichia stipitis Using an EGFP Reporter Gene V. Passoth and U. Klinner and B. Hahn-Hagerdahl p. 241
Chapter 39 Cyanide-Insensitive Respiration A. Eliasson and V. Passoth and B. Hahn-Hagerdal p. 245
Chapter 40 Freeze Transformation, Plasmid Reisolation and Stability in Pichia stipitis V. Passoth and B. Hahn-Hagerdal and U. Klinner p. 253
Schwanniomyces occidentalis
Chapter 41 Objective Analysis of Medium-Induced Fragility of Schwanniomyces occidentalis BF. Johnson and D. Wahalawatta and S. Mukherjee and C. Ficker and S. Boroumandi and O. Clarkin and B. Ramsingh and F. Siddiqi and M. Vessal and J. Escorcia and E. Chock and M. Lopez and S. Khulbe and H. Privora and R. Booth and GB. Calleja p. 263
Chapter 42 Subjective Analysis of Medium-Induced Fragility of Schwanniomyces occidentalis BF. Johnson and GB. Calleja and T. Walker p. 267
Chapter 43 Analysis of Structural Discontinuities of the Wall of Schwanniomyces occidentalis BF. Johnson and GB. Calleja and B-Y. Yoo and K. Liaquat p. 271
Chapter 44 Induction of Amylases in Schwanniomyces occidentalis GB. Calleja and T. Walker and S. Levy-Rick and BF. Johnson p. 275
Chapter 45 Ethanol Production from Starch by Schwanniomyces occidentalis GB. Calleja and S. Levy-Rick and T. Walker and BF. Johnson p. 279
Trichosporon domesticum
Chapter 46 Characterization of Soluble Cytochrome P450 from Trichosporon domesticum H. Laternicht and F. Schauer p. 285
Trichosporon montevideense
Chapter 47 Oxidation of the Fungicide Biphenyl by Yeasts of the Genus Trichosporon E. Hammer and F. Schauer p. 293
Xanthophyllomyces dendroorhous
Chapter 48 Genetic Complementation Analysis by Protoplast Fusion of Xanthophyllomyces dendrorhous P. Retamales and V. Cifuentes p. 299
Chapter 49 Lethal Effect of UV Light and Photoreactivation in Xanthophyllomyces dendrorhous P. Retamales and V. Cifuentes p. 305
Chapter 50 Fermentation and Carotenoid Analysis of the Yeast Xanthophyllomyces dendrorhous (Phaffia rhodozyma) H. Visser and JC. Verdoes and AJJ. van Ooyen p. 309
Chapter 51 Metabolic Engineering of the Carotenoid Biosynthetic Pathway in Xanthophyllomyces dendrorhous (Phaffia rhodozyma) JC. Verdoes and H. Visser and AJJ. van Ooyen p. 315
Chapter 52 Astaxanthin Biosynthesis in Xanthophyllomyces dendrorhous (Phaffia rhodozyma): Effect of Inhibitors and Low Temperature LM. Ducrey Santopietro p. 323
Chapter 53 Purification and Characterization of Extrachromosomal Genetic Elements of Double-Stranded RNA (dsRNA) of Xanthophyllomyces dendrorhous A. Castillo and V. Cifuentes p. 329
Yarrowia lipolytica
Chapter 54 Mating, Sporulation and Random Spore Selection by Nystatin in Yarrowia lipolytica G. Barth p. 335
Chapter 55 Induction of the Yeast-Hypha Transition in Yarrowia lipolytica A. Dominguez p. 339
Chapter 56 Tagging of Genes by Insertional Mutagenesis in the Yeast Yarrowia lipolytica S. Mauersberger and J-M. Nicaud p. 343
Chapter 57 Detection and Activation of the Ylt1 Retrotransposon in the Yeast Yarrowia lipolytica S. Senam and G. Barth p. 357
Chapter 58 Flow Cytometry of Yarrowia lipolytica A. Dominguez p. 365
Chapter 59 A Copper-Regulated Yarrowia lipolytica Vector for the Expression of Heterologous Proteins A. Dominguez and M. Prado and S. Garcia p. 369
Chapter 60 Lipase Assay in Yarrowia lipolytica M. Morin and FJ. Gonzalez and A. Choupina and FJ. Burguillo and A. Dominguez p. 373
Chapter 61 Expression of Mutant Gpr1p in Yarrowia lipolytica and Its Effect on Growth on Different Media M. Gentsch and M. Kuschel and G. Barth p. 379
Chapter 62 Detection of Biosynthesis and Degradation of Peroxisomes by Different Carbon Sources with a Combined Assay Using a [beta]-Galactosidase-Gfp-SKL Chimaeric Protein for Labeling of Peroxisomes and the Peroxisomal Isocitrate Lyase as Control|cI. Parshyna G. Barth p. 385
Chapter 63 Induction of Citric Acid/Isocitric Acid and [alpha]-Ketoglutaric Acid Production in the Yeast Yarrowia lipolytica S. Mauersberger and K. Kruse and G. Barth p. 393
Chapter 64 Regulation of Production of Yarrowia lipolytica Extracellular Ribonuclease and Alkaline Extracellular Protease DM. Ogrydziak p. 401
Zygosaccharomyces bailii
Chapter 65 Zygosaccharomyces bailii: A Yeast with a Peculiar Pattern for the Regulation of Acetic Acid Metabolism in the Presence of Glucose F. Rodrigues and MJ. Sousa and M. Corte-Real and C. Leao p. 409
Chapter 66 Transfection of Yeast with Toxin-Encoding dsRNA Viruses of Zygosaccharomyces bailii and Toxin Purification by Receptor-Mediated Affinity Chromatography F. Weiler and MJ. Schmitt p. 417
Zygosaccharomyces rouxii
Chapter 67 Heat-Shock-Induced Activation of Trehalase in the Yeast Zygosaccharomyces rouxii T. Soto and J. Vicente-Soler and J. Cansado and M. Gacto p. 429
Miscellaneous Non-Conventional Yeasts
Chapter 68 Linear and Circular Plasmids of Yeasts H. Fukuhara p. 437
Chapter 69 Rapid Method to Extract DNA from Capsulated Yeast Species G. Cardinali and A. Bolano and F. Fatichenti p. 445
Chapter 70 Testing Various Yeasts for Ethanol Production from Xylose GB. Calleja and S. Levy-Rick and BF. Johnson p. 451
Chapter 71 Use of a Differential Culture Medium for the Enumeration of Zygosaccharomyces bailii, Saccharomyces cerevisiae and Pichia membranifaciens in Wine D. Schuller and M. Corte-Real and C. Leao p. 457
Chapter 72 Characterization of Ubiquinone Type (CoQ7-CoQ10) of Non-Conventional Yeasts F. Schauer and E. Hammer p. 463
Chapter 73 Non-Radioactive Dot Blot DNA Reassociation for Unequivocal Yeast Identification G. Cardinali and G. Liti and F. Fatichenti p. 469
Chapter 74 Taxonomy Rules and Classification Hints for Non-Taxonomists Working with Unknown or Ill-Identified Yeast Cultures A. Martini and AE. Vaughan and G. Cardinali p. 475
Subject Index p. 491