Methods in gene technology A research annual

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作   者:Jeremy W. Dale, Peter G. Sanders

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ISBN:9781559382632

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简介

目录

List of Contributors
Introduction
Purification of DNA J.W. Dale
Electrophoretic Separation of Nucleic Acids P.G. Sanders
Preparative Gel Electrophoresis J.C. Maule
Plasmid Isolation Procedures A.I. Knight
Plasmid Isolation: column Methods T.J. Hellyer
Isolation of Large Plasmids G.L. Jones and P.A. Williams
Isolation of Linear and Single-Strand Plasmid DNA J.C. Alonso
Preparation of Bacteriophage DNA E. Norman
Purification of Viral RNA J.E. Whitby and A.D.T. Barrett and A.D. Jennings and P. Johnstone
Large Scale DNA Sequencing by Manual Methods A.J. Davison and E.A.R. Telford
Pulsed-Field Gel Electrophoresis: Applications to Bacteria P.R. Stewart and W. El-Adhami and B. Inglis
Pulsed-Field Gel Electrophoresis: Large Scale Restriction Maps N. Okahashi and T. Koga
Pulsed-Field Gel Electrophoresis: Isolation and Analysis of Large Linear Plasmids H. Kinashi
Detection of Mutations by PCR-Single-Strand Conformation Polymorphism A. Telenti
Analysis of DNA Supercoiling K. Drlica and M. Malik and J.Y. Wang and A. Sasiak and R.R. Sinden
Gel Retardation Assays and DNA Footprinting I.S. Goping and D.A. Underhill and G.C. Shore
Southwestern Blot G. Corte and M.T. Corsetti
Genomic Fingerprinting Using the PCR-Random Amplified Polymorphic DNA Technique S.V.P.S. Silva and J.D. Procunier
PCR Walking M.G. Burdon and J.J. Willoughby
Index

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