简介
Summary:
Publisher Summary 1
Scientists from around the world share laboratory protocols they use to study the disease characterized by reduced bone mass, quality, and strength. They include in vitro models to study bone cells and tissue, in vivo rodent models, gene delivery, molecular and genetic techniques, imaging techniques, and mechanical testing. The disk, which requires one of the major proprietary operating systems, contains color reproductions of illustrations in the text. Annotation 漏2008 Book News, Inc., Portland, OR (booknews.com)
Publisher Summary 2
The study of bone cells and tissues at the cellular and molecular levels in a variety of models has revolutionized the field. In this book, leading scientists from around the world share their step-by-step laboratory protocols for studying bone biology.聽This is the perfect guide for both new and experienced experimentalists attempting to study osteoporosis.
目录
List of Color Plates p. xi
Part I In Vitro Models
1 Isolation and Culture of Rodent Osteoprogenitor Cells Thomas A. Owen and Lydia C. Pan p. 3
2 Osteoclast Culture and Resorption Assays Elizabeth W. Bradley and Merry Jo Oursler p. 19
3 Assessing New Bone Formation in Neonatal Calvarial Organ Cultures Khalid S. Mohammad and John M. Chirgwin and Theresa A. Guise p. 37
4 Detection of Apoptosis of Bone Cells In Vitro Teresita Bellido and Lilian I. Plotkin p. 51
Part II In Vivo Rodent Models
5 In Vivo Parathyroid Hormone Treatments and RNA Isolation and Analysis Xin Li and Ling Qin and Nicola C. Partridge p. 79
6 Assessment of Bone Formation Capacity Using In vivo Transplantation Assays: Procedure and Tissue Analysis Basem M. Abdallah and Nicholas Ditzel and Moustapha Kassem p. 89
7 Ossicle and Vossicle Implant Model Systems Glenda J. Pettway and Laurie K. McCauley p. 101
8 Utility of the Ovariectomized Rat as a Model for Human Osteoporosis in Drug Discovery Yogendra P. Kharode and Michael C. Sharp and Peter V.N. Bodine p. 111
9 Orchidectomy Models of Osteoporosis Stephane Blouin and Helene Libouban and Marie Francoise Moreau and Daniel Chappard p. 125
Part III Methods of Gene Delivery
10 Gene Delivery by Adenoviruses Renny T. Franceschi and Chunxi Ge p. 137
11 Lentivirus Delivery of shRNA Constructs into Osteoblasts Marc N. Wein and Dallas C. Jones and Laurie H. Glimcher p. 149
12 Gene Delivery by Retroviruses Valerie Deregowski and Ernesto Canalis p. 157
Part IV Molecular and Genetic Techniques
13 Chromatin Immunoprecipitation Assays: Application of ChIP-on-Chip for Defining Dynamic Transcriptional Mechanisms in Bone Cells Margaretha van der Deen and Mohammad Q. Hassan and Jitesh Pratap and Nadiya M. Teplyuk and Daniel W. Young and Amjad Javed and Sayyed K. Zaidi and Jane B. Lian and Martin Montecino and Janet L. Stein and Gary S. Stein and Andre J. van Wijnen p. 165
14 Identification of Transcription Factor Target Genes by ChIP Display Artem Barski and Steven Pregizer and Baruch Frenkel p. 177
15 Application of the Laser Capture Microdissection Technique for Molecular Definition of Skeletal Cell Differentiation In Vivo Dafna Benayahu and Rina Socher and Irena Shur p. 191
16 Quantitative Trait Loci Mapping Dong-Hai Xiong and Jian-Feng Liu and Yan-Fang Guo and Yan Guo and Tie-Lin Yang and Hui Jiang and Yuan Chen and Fang Yang and Robert R Recker and Hong-Wen Deng p. 203
Part V Imaging Techniques
17 In Situ Nuclear Organization of Regulatory Machinery Shirwin M. Pockwinse and Sayyed K. Zaidi and Ricardo F. Medina and Rachit Bakshi and Krishna P. Kota and Syed A. Ali and Daniel W. Young and Jeffery A. Nickerson and Amjad Javed and Martin Montecino and Andre J. van Wijnen and Jane B. Lian and Janet L. Stein and Gary S. Stein p. 239
18 Bioluminescent Imaging in Bone Yoram Zilberman and Yossi Gafni and Gadi Pelled and Zulma Gazit and Dan Gazit p. 261
19 Micro-Computed Tomography: A Method for the Non-Destructive Evaluation of the Three-Dimensional Structure of Biological Specimens Martin Stauber and Ralph Muller p. 273
20 Fourier Transform-Infrared Microspectroscopy and Microscopic Imaging Samuel Gourion-Arsiquaud and Paul A. West and Adele L. Boskey p. 293
Part VI Mechanical Testing
21 Assessment of the In Vivo Adaptive Response to Mechanical Loading Leanne Kaye Saxon and Lance Edward Lanyon p. 307
22 Determination of Bone's Mechanical Matrix Properties by Nanoindentation Engin Ozcivici and Suzanne Ferreri and Yi-Xian Qin and Stefan Judex p. 323
23 Fluid Flow Assays Ryan C. Riddle and Amanda F. Taylor and Henry J. Donahue p. 335
Index p. 347
Part I In Vitro Models
1 Isolation and Culture of Rodent Osteoprogenitor Cells Thomas A. Owen and Lydia C. Pan p. 3
2 Osteoclast Culture and Resorption Assays Elizabeth W. Bradley and Merry Jo Oursler p. 19
3 Assessing New Bone Formation in Neonatal Calvarial Organ Cultures Khalid S. Mohammad and John M. Chirgwin and Theresa A. Guise p. 37
4 Detection of Apoptosis of Bone Cells In Vitro Teresita Bellido and Lilian I. Plotkin p. 51
Part II In Vivo Rodent Models
5 In Vivo Parathyroid Hormone Treatments and RNA Isolation and Analysis Xin Li and Ling Qin and Nicola C. Partridge p. 79
6 Assessment of Bone Formation Capacity Using In vivo Transplantation Assays: Procedure and Tissue Analysis Basem M. Abdallah and Nicholas Ditzel and Moustapha Kassem p. 89
7 Ossicle and Vossicle Implant Model Systems Glenda J. Pettway and Laurie K. McCauley p. 101
8 Utility of the Ovariectomized Rat as a Model for Human Osteoporosis in Drug Discovery Yogendra P. Kharode and Michael C. Sharp and Peter V.N. Bodine p. 111
9 Orchidectomy Models of Osteoporosis Stephane Blouin and Helene Libouban and Marie Francoise Moreau and Daniel Chappard p. 125
Part III Methods of Gene Delivery
10 Gene Delivery by Adenoviruses Renny T. Franceschi and Chunxi Ge p. 137
11 Lentivirus Delivery of shRNA Constructs into Osteoblasts Marc N. Wein and Dallas C. Jones and Laurie H. Glimcher p. 149
12 Gene Delivery by Retroviruses Valerie Deregowski and Ernesto Canalis p. 157
Part IV Molecular and Genetic Techniques
13 Chromatin Immunoprecipitation Assays: Application of ChIP-on-Chip for Defining Dynamic Transcriptional Mechanisms in Bone Cells Margaretha van der Deen and Mohammad Q. Hassan and Jitesh Pratap and Nadiya M. Teplyuk and Daniel W. Young and Amjad Javed and Sayyed K. Zaidi and Jane B. Lian and Martin Montecino and Janet L. Stein and Gary S. Stein and Andre J. van Wijnen p. 165
14 Identification of Transcription Factor Target Genes by ChIP Display Artem Barski and Steven Pregizer and Baruch Frenkel p. 177
15 Application of the Laser Capture Microdissection Technique for Molecular Definition of Skeletal Cell Differentiation In Vivo Dafna Benayahu and Rina Socher and Irena Shur p. 191
16 Quantitative Trait Loci Mapping Dong-Hai Xiong and Jian-Feng Liu and Yan-Fang Guo and Yan Guo and Tie-Lin Yang and Hui Jiang and Yuan Chen and Fang Yang and Robert R Recker and Hong-Wen Deng p. 203
Part V Imaging Techniques
17 In Situ Nuclear Organization of Regulatory Machinery Shirwin M. Pockwinse and Sayyed K. Zaidi and Ricardo F. Medina and Rachit Bakshi and Krishna P. Kota and Syed A. Ali and Daniel W. Young and Jeffery A. Nickerson and Amjad Javed and Martin Montecino and Andre J. van Wijnen and Jane B. Lian and Janet L. Stein and Gary S. Stein p. 239
18 Bioluminescent Imaging in Bone Yoram Zilberman and Yossi Gafni and Gadi Pelled and Zulma Gazit and Dan Gazit p. 261
19 Micro-Computed Tomography: A Method for the Non-Destructive Evaluation of the Three-Dimensional Structure of Biological Specimens Martin Stauber and Ralph Muller p. 273
20 Fourier Transform-Infrared Microspectroscopy and Microscopic Imaging Samuel Gourion-Arsiquaud and Paul A. West and Adele L. Boskey p. 293
Part VI Mechanical Testing
21 Assessment of the In Vivo Adaptive Response to Mechanical Loading Leanne Kaye Saxon and Lance Edward Lanyon p. 307
22 Determination of Bone's Mechanical Matrix Properties by Nanoindentation Engin Ozcivici and Suzanne Ferreri and Yi-Xian Qin and Stefan Judex p. 323
23 Fluid Flow Assays Ryan C. Riddle and Amanda F. Taylor and Henry J. Donahue p. 335
Index p. 347
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