微信扫一扫,移动浏览光盘
简介
Summary:
Publisher Summary 1
Provides material lists and step-by-step instructions for conducting proteomic experiments in the laboratory, particularly the identification of proteins by mass spectroscopy. The 34 chapters describe general techniques, post-translation modifications, variant separations, protein isoforms, methods for studying specific systems, and tools for data analysis. Topics include multicolor detection on Western blots, a strategy for characterizing antibody/antigen interactions using ProteinChip arrays, stable isotope labeling with amino acids, predicting glycan composition from experimental mass, quantitative characterization of proteomics maps by matrix invaritants, and patchwork peptide sequencing. Annotation (c) Book News, Inc., Portland, OR (booknews.com)
Publisher Summary 2
A compendium of thirty-four powerful techniques for identifying and analyzing the diversity of proteins expressed in cells. Thee readily reproducible proteomic methods range from general to specific techniques, and include methods for data analysis, posttranslational modification, and its variants and isoforms. Additional methods demonstrate the application of proteomics to the discovery of serological tumor markers, to identifying the determinants of sensitivity to antitumor drugs, and to specialized fields, such as endocrinology, plant biology, nephrology, and urology.
目录
Preface p. v
Contributors p. xi
Part I General Techniques
1 Proteomics and the Molecular Biologist Paul R. Graves and Timothy A. J. Haystead p. 3
2 Protein Identification from 2-D Gels Using In Vitro Transcription Translation Products Nathalie Norais and Renzo Nogarotto and Emilia Tiziana Iacobini and Ignazio Garaguso and Renata Grifantini and Giuliano Galli and Guido Grandi p. 17
3 Selective Chemical Cleavage Methods in Proteomics, Including C-Terminal Successive Degradation Akira Tsugita p. 29
4 Means of Hydrolyzing Proteins Isolated upon ProteinChip Array Surfaces: Chemical and Enzymatic Approaches Shanhua Lin and Ning Tang and Scot R. Weinberger p. 59
5 A Combined Radiolabeling and Silver Staining Technique for Improved Visualization and Localization of Proteins on Two-Dimensional Gels Jules A. Westbrook and Michael J. Dunn p. 73
6 Qualitative and Quantitative Proteomic Analyses via Multidimensional Protein Identification Technology Michael P. Washburn and David M. Schieltz p. 79
7 Di- and Tri-Chromatic Fluorescence Detection on Western Blots Karen J. Martin and Wayne F. Patton p. 93
8 Multiplexed Proteomics: Fluorescence Detection of Protein Differences by Two-Dimensional Gel Electrophoresis and Computer-Based Differential Display Birte Schulenberg and Wayne F. Patton p. 107
9 A Strategy for Characterizing Antibody/Antigen Interactions Using ProteinChip Arrays Alexandra Huhalov and Daniel I. R. Spencer and Kerry A. Chester p. 117
10 Stable Isotope Labeling with Amino Acids as an Aid to Protein Identification in Peptide Mass Fingerprinting Robert J. Beynon p. 129
11 The Use of [superscript 18]O Labeling as a Tool for Proteomic Applications Ian I. Stewart and Ty Thomson and Daniel Figeys and Henry S. Duewel p. 145
12 Automated Nanoflow Liquid Chromatography/Tandem Mass Spectrometric Identification of Liver Mitochondrial Proteins Bart Devreese and Frank Vanrobaeys and Elke Lecocq and Joel Smet and Rudy Van Coster and Jozef Van Beeumen p. 181
13 In Silico Proteomics: Predicting Interactions from Sequence Joel R. Bock and David A. Gough p. 193
Part II Post-Translational Modifications, Variants, and Isoforms
14 Predicting Glycan Composition from Experimental Mass Using GlycoMod Catherine A. Cooper and Elisabeth Gasteiger and Nicolle H. Packer p. 225
15 Querying GlycoSuiteDB Catherine A. Cooper and Hiren J. Joshi and Mathew J. Harrison and Marc R. Wilkins and Nicolle H. Packer p. 233
16 New Tools for Quantitative Phosphoproteome Analysis Li-Rong Yu and Van M. Hoang and Timothy D. Veenstra p. 241
17 Computer-Aided Strategies for Characterizing Protein Isoforms Frederic Nikitin and Frederique Lisacek p. 259
18 Protein Variant Separations Using Cation Exchange Chromatography on Grafted, Polymeric Stationary Phases Michael Weitzhandler and Dell Farnan and Nebojsa Avdalovic and Chris Pohl p. 269
Part III Specific Systems
19 Noninvasive Imaging of Protein-Protein Interactions in Living Animals Gary D. Luker and Vijay Sharma and David Piwnica-Worms p. 283
20 Strategies in Clinical Proteomics Eric T. Fung p. 299
21 Proteomic Profiling of the Cancer Microenvironment Vladimir Knezevic and Michael R. Emmert-Buck p. 309
22 Identification of Determinants of Sensitivity to Antitumor Drugs Paola Perego and Giovanni Luca Beretta and Laura Gatti p. 319
23 Application of Proteomics to the Discovery of Serological Tumor Markers Terence C. W. Poon and Philip J. Johnson p. 331
24 Infectomic Analysis of Microbial Infections Using Proteomics Sheng-He Huang and Ambrose Jong and James T. Summersgill p. 347
25 Toward a Complete Proteome of Bacillus subtilis: Cytosolic, Cell Wall-Associated, and Extracellular Proteins Haike Antelmann and Jan Maarten van Dijl and Michael Hecker p. 357
26 Renal and Urinary Proteomics Visith Thongboonkerd and Elias Klein and Jon B. Klein p. 375
27 Proteomics in Endocrinology Jan W. A. Smit and Johannes A. Romijn p. 397
28 Proteomics in Plant Biology Christina Mihr and Hans-Peter Braun p. 409
Part IV Data Analysis
29 Bioinformatics in Proteomics Mauno Vihinen p. 419
30 Quantitative Characterization of Proteomics Maps by Matrix Invariants Milan Randic p. 429
31 Complexity of Protein-Protein Interaction Networks, Complexes, and Pathways Danail Bonchev p. 451
32 Patchwork Peptide Sequencing: Protein Identification by Accurate Mass-to-Sequence Conversion of High-Resolution Q-TOF Tandem Mass Spectrometry Data Andreas Schlosser and Wolf D. Lehmann p. 463
33 Estimation of Bias in Proteome Research Ralf Mrowka and Hanspeter Herzel p. 473
34 Scoring Functions for Mass Spectrometric Protein Identification Robin Gras and Patricia Hernandez and Markus Muller and Ron D. Appel p. 477
Index p. 487
Contributors p. xi
Part I General Techniques
1 Proteomics and the Molecular Biologist Paul R. Graves and Timothy A. J. Haystead p. 3
2 Protein Identification from 2-D Gels Using In Vitro Transcription Translation Products Nathalie Norais and Renzo Nogarotto and Emilia Tiziana Iacobini and Ignazio Garaguso and Renata Grifantini and Giuliano Galli and Guido Grandi p. 17
3 Selective Chemical Cleavage Methods in Proteomics, Including C-Terminal Successive Degradation Akira Tsugita p. 29
4 Means of Hydrolyzing Proteins Isolated upon ProteinChip Array Surfaces: Chemical and Enzymatic Approaches Shanhua Lin and Ning Tang and Scot R. Weinberger p. 59
5 A Combined Radiolabeling and Silver Staining Technique for Improved Visualization and Localization of Proteins on Two-Dimensional Gels Jules A. Westbrook and Michael J. Dunn p. 73
6 Qualitative and Quantitative Proteomic Analyses via Multidimensional Protein Identification Technology Michael P. Washburn and David M. Schieltz p. 79
7 Di- and Tri-Chromatic Fluorescence Detection on Western Blots Karen J. Martin and Wayne F. Patton p. 93
8 Multiplexed Proteomics: Fluorescence Detection of Protein Differences by Two-Dimensional Gel Electrophoresis and Computer-Based Differential Display Birte Schulenberg and Wayne F. Patton p. 107
9 A Strategy for Characterizing Antibody/Antigen Interactions Using ProteinChip Arrays Alexandra Huhalov and Daniel I. R. Spencer and Kerry A. Chester p. 117
10 Stable Isotope Labeling with Amino Acids as an Aid to Protein Identification in Peptide Mass Fingerprinting Robert J. Beynon p. 129
11 The Use of [superscript 18]O Labeling as a Tool for Proteomic Applications Ian I. Stewart and Ty Thomson and Daniel Figeys and Henry S. Duewel p. 145
12 Automated Nanoflow Liquid Chromatography/Tandem Mass Spectrometric Identification of Liver Mitochondrial Proteins Bart Devreese and Frank Vanrobaeys and Elke Lecocq and Joel Smet and Rudy Van Coster and Jozef Van Beeumen p. 181
13 In Silico Proteomics: Predicting Interactions from Sequence Joel R. Bock and David A. Gough p. 193
Part II Post-Translational Modifications, Variants, and Isoforms
14 Predicting Glycan Composition from Experimental Mass Using GlycoMod Catherine A. Cooper and Elisabeth Gasteiger and Nicolle H. Packer p. 225
15 Querying GlycoSuiteDB Catherine A. Cooper and Hiren J. Joshi and Mathew J. Harrison and Marc R. Wilkins and Nicolle H. Packer p. 233
16 New Tools for Quantitative Phosphoproteome Analysis Li-Rong Yu and Van M. Hoang and Timothy D. Veenstra p. 241
17 Computer-Aided Strategies for Characterizing Protein Isoforms Frederic Nikitin and Frederique Lisacek p. 259
18 Protein Variant Separations Using Cation Exchange Chromatography on Grafted, Polymeric Stationary Phases Michael Weitzhandler and Dell Farnan and Nebojsa Avdalovic and Chris Pohl p. 269
Part III Specific Systems
19 Noninvasive Imaging of Protein-Protein Interactions in Living Animals Gary D. Luker and Vijay Sharma and David Piwnica-Worms p. 283
20 Strategies in Clinical Proteomics Eric T. Fung p. 299
21 Proteomic Profiling of the Cancer Microenvironment Vladimir Knezevic and Michael R. Emmert-Buck p. 309
22 Identification of Determinants of Sensitivity to Antitumor Drugs Paola Perego and Giovanni Luca Beretta and Laura Gatti p. 319
23 Application of Proteomics to the Discovery of Serological Tumor Markers Terence C. W. Poon and Philip J. Johnson p. 331
24 Infectomic Analysis of Microbial Infections Using Proteomics Sheng-He Huang and Ambrose Jong and James T. Summersgill p. 347
25 Toward a Complete Proteome of Bacillus subtilis: Cytosolic, Cell Wall-Associated, and Extracellular Proteins Haike Antelmann and Jan Maarten van Dijl and Michael Hecker p. 357
26 Renal and Urinary Proteomics Visith Thongboonkerd and Elias Klein and Jon B. Klein p. 375
27 Proteomics in Endocrinology Jan W. A. Smit and Johannes A. Romijn p. 397
28 Proteomics in Plant Biology Christina Mihr and Hans-Peter Braun p. 409
Part IV Data Analysis
29 Bioinformatics in Proteomics Mauno Vihinen p. 419
30 Quantitative Characterization of Proteomics Maps by Matrix Invariants Milan Randic p. 429
31 Complexity of Protein-Protein Interaction Networks, Complexes, and Pathways Danail Bonchev p. 451
32 Patchwork Peptide Sequencing: Protein Identification by Accurate Mass-to-Sequence Conversion of High-Resolution Q-TOF Tandem Mass Spectrometry Data Andreas Schlosser and Wolf D. Lehmann p. 463
33 Estimation of Bias in Proteome Research Ralf Mrowka and Hanspeter Herzel p. 473
34 Scoring Functions for Mass Spectrometric Protein Identification Robin Gras and Patricia Hernandez and Markus Muller and Ron D. Appel p. 477
Index p. 487
Handbook of proteomic methods /
- 名称
- 类型
- 大小
光盘服务联系方式: 020-38250260 客服QQ：4006604884
云图客服：
用户发送的提问，这种方式就需要有位在线客服来回答用户的问题，这种 就属于对话式的,问题是这种提问是否需要用户登录才能提问
Video Player
×
Audio Player
×
pdf Player
×
