Principles and reactions of protein extraction, purification, and characterization /
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作 者：Hafiz Ahmed.
分类号：
ISBN：9780849320347
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简介
Principles and Reactions of Protein Extraction, Purification, and Characterization provides the mechanisms and experimental procedures for classic to cutting-edge techniques used in protein extraction, purification, and characterization. The author presents the principles and reactions behind each procedure and uses tables to compare the different methods. The book also discusses the development of antibodies and immunochemical techniques as tools for characterizing proteins and modified proteins such as glycoproteins. Helpful illustrations, diagrams, and tables effectively transform theoretical concepts into practical knowledge. Along with methodical working procedures for most techniques, the book also offers useful advice on which technique to use and when to apply a particular method. (Midwest)
目录
Front cover 1
Foreword 8
Preface 10
Profile of the Author 12
Contents 14
Chapter 1. Extraction of Protein 22
Chapter 2. Estimation of Protein 56
Chapter 3. Electrophoretic Analyses of Protein 92
Chapter 4. Purification of Protein 154
Chapter 5. Antibodies: Structures, Interactions, and Production 214
Chapter 6. Antibody Labeling, Antibody Detection, and Immunochemical Techniques 258
Chapter 7. Purification of Glycoproteins and Analyses of their Oligosaccharides 358
Index 400
Back cover 410
Foreword 8
Preface 10
Profile of the Author 12
Contents 14
Chapter 1. Extraction of Protein 22
Chapter 2. Estimation of Protein 56
Chapter 3. Electrophoretic Analyses of Protein 92
Chapter 4. Purification of Protein 154
Chapter 5. Antibodies: Structures, Interactions, and Production 214
Chapter 6. Antibody Labeling, Antibody Detection, and Immunochemical Techniques 258
Chapter 7. Purification of Glycoproteins and Analyses of their Oligosaccharides 358
Index 400
Back cover 410
Principles and reactions of protein extraction, purification, and characterization /
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