DNA amplification : current technologies and applications /
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作 者：edited by: Vadim V. Demidov and Natalia E. Broude.
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ISBN：9780954523299
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简介
Seeking to go beyond works that discuss merely one form of DNA amplification (polymerase chain reaction) or look at amplification for the purpose of narrow goals, Demidov and Broude (both of the Center for Advanced Biotechnology, Boston U.) present 17 papers discussing a wide range of amplification techniques and applications in an attempt to present the current state-of-the-art in "the cornerstone of modern biotechnology." Four sections explore enzymes used in DNA amplification, thermocycling methods of DNA amplification, isothermal methods of DNA amplification, and DNA amplification in detection of non-DNA analytes. Distributed by Taylor & Francis. Annotation: 2004 Book News, Inc., Portland, OR (booknews.com)
目录
Contents p. iii
Contributors p. v
Preface p. ix
Section 1. Enzymes Used in DNA Amplification
Chapter 1.1 Thermostable Chimeric DNA Polymerases with High Resistance to Inhibitors Andrey R. Pavlov and Nadejda V. Pavlova and Sergei A. Kozyavkin and Alexei I. Slesarev p. 3
Chapter 1.2 Phi29 DNA Polymerase, a Potent Amplification Enzyme Margarita Salas and Miguel de Vega and Jose M. Lazaro and Luis Blanco p. 21
Chapter 1.3 High-Fidelity Thermostable DNA Ligases as a Tool for DNA Amplification Weiguo Cao p. 35
Section 2. Thermocycling Methods of DNA Amplification
Chapter 2.1 High Multiplexity PCR Based on PCR Suppression Natalia E. Broude and Adriaan W. van Heusden and Richard Finkers p. 61
Chapter 2.2 On-Chip PCR: DNA Amplification and Analysis on Oligonucleotide Microarrays Martin Huber and Christian Harwanegg and Manfred W. Mueller and Wolfgang M. Schmidt p. 77
Chapter 2.3 Analysis of Somatic Mutations via Long-Distance Single Molecule PCR Yevgenya Kraytsberg and Ekaterina Nekhaeva and Connie Chang and Konstantin Ebralidse and Konstantin Khrapko p. 97
Chapter 2.4 Digital PCR Analysis of Allelic Status in Clinical Specimens Wei Zhou and Tanisha Williams and Cecile Colpaert and Aki Morikawa and Diansheng Zhong p. 111
Chapter 2.5 Real-Time Quantitative PCR in the Analysis of Gene Expression Manohar R. Furtado and Olga V. Petrauskene and Kenneth J. Livak p. 131
Chapter 2.6 Quantitative Genetic Analysis with Multiplex Ligation-Dependent Probe Amplification (MLPA) A.O.H Nygren and A. Errami and J.P. Schouten p. 147
Section 3. Isothermal Methods of DNA Amplification
Chapter 3.1 Homogeneous Real-Time Strand Displacement Amplification David M. Wolfe and Sha-Sha Wang and Keith Thornton and Andrew M. Kuhn and James G. Nadeau and Tobin J. Hellyer p. 173
Chapter 3.2 Loop-Mediated Isothermal Amplification (LAMP) of DNA Analytes Tsugunori Notomi and Kentaro Nagamine and Yasuyoshi Mori and Hidetoshi Kanda p. 199
Chapter 3.3 Ligation-Mediated Rolling Circle DNA Amplification for Non-Gel Detection of Single Nucleotide Polymorphisms (SNPs) Xiaoquan Qi p. 213
Chapter 3.4 Rolling-Circle Amplification of Duplex DNA Sequences Assisted by PNA Openers Heiko Kuhn and Vadim V. Demidov p. 227
Chapter 3.5 Phi29 DNA Polymerase Based Rolling Circle Amplification of Templates for DNA Sequencing John C. Detter and John R. Nelson and Paul M. Richardson p. 245
Chapter 3.6 Multiple-Displacement Amplification (MDA) of Whole Human Genomes from Various Samples Roger S. Lasken and Seiyu Hosono and Michael Egholm p. 267
Section 4. DNA Amplification in Detection of Non-DNA Analytes
Chapter 4.1 Enhanced Protein Detection Using Real-Time Immuno-PCR Michael Adler and Christof M. Niemeyer p. 293
Chapter 4.2 Rolling Circle Amplification in Multiplex Immunoassays Michael C. Mullenix and Richard S. Dondero and Hirock D. Datta and Michael Egholm and Stephen F. Kingsmore and Lorah T. Perlee p. 313
Index p. 333
Contributors p. v
Preface p. ix
Section 1. Enzymes Used in DNA Amplification
Chapter 1.1 Thermostable Chimeric DNA Polymerases with High Resistance to Inhibitors Andrey R. Pavlov and Nadejda V. Pavlova and Sergei A. Kozyavkin and Alexei I. Slesarev p. 3
Chapter 1.2 Phi29 DNA Polymerase, a Potent Amplification Enzyme Margarita Salas and Miguel de Vega and Jose M. Lazaro and Luis Blanco p. 21
Chapter 1.3 High-Fidelity Thermostable DNA Ligases as a Tool for DNA Amplification Weiguo Cao p. 35
Section 2. Thermocycling Methods of DNA Amplification
Chapter 2.1 High Multiplexity PCR Based on PCR Suppression Natalia E. Broude and Adriaan W. van Heusden and Richard Finkers p. 61
Chapter 2.2 On-Chip PCR: DNA Amplification and Analysis on Oligonucleotide Microarrays Martin Huber and Christian Harwanegg and Manfred W. Mueller and Wolfgang M. Schmidt p. 77
Chapter 2.3 Analysis of Somatic Mutations via Long-Distance Single Molecule PCR Yevgenya Kraytsberg and Ekaterina Nekhaeva and Connie Chang and Konstantin Ebralidse and Konstantin Khrapko p. 97
Chapter 2.4 Digital PCR Analysis of Allelic Status in Clinical Specimens Wei Zhou and Tanisha Williams and Cecile Colpaert and Aki Morikawa and Diansheng Zhong p. 111
Chapter 2.5 Real-Time Quantitative PCR in the Analysis of Gene Expression Manohar R. Furtado and Olga V. Petrauskene and Kenneth J. Livak p. 131
Chapter 2.6 Quantitative Genetic Analysis with Multiplex Ligation-Dependent Probe Amplification (MLPA) A.O.H Nygren and A. Errami and J.P. Schouten p. 147
Section 3. Isothermal Methods of DNA Amplification
Chapter 3.1 Homogeneous Real-Time Strand Displacement Amplification David M. Wolfe and Sha-Sha Wang and Keith Thornton and Andrew M. Kuhn and James G. Nadeau and Tobin J. Hellyer p. 173
Chapter 3.2 Loop-Mediated Isothermal Amplification (LAMP) of DNA Analytes Tsugunori Notomi and Kentaro Nagamine and Yasuyoshi Mori and Hidetoshi Kanda p. 199
Chapter 3.3 Ligation-Mediated Rolling Circle DNA Amplification for Non-Gel Detection of Single Nucleotide Polymorphisms (SNPs) Xiaoquan Qi p. 213
Chapter 3.4 Rolling-Circle Amplification of Duplex DNA Sequences Assisted by PNA Openers Heiko Kuhn and Vadim V. Demidov p. 227
Chapter 3.5 Phi29 DNA Polymerase Based Rolling Circle Amplification of Templates for DNA Sequencing John C. Detter and John R. Nelson and Paul M. Richardson p. 245
Chapter 3.6 Multiple-Displacement Amplification (MDA) of Whole Human Genomes from Various Samples Roger S. Lasken and Seiyu Hosono and Michael Egholm p. 267
Section 4. DNA Amplification in Detection of Non-DNA Analytes
Chapter 4.1 Enhanced Protein Detection Using Real-Time Immuno-PCR Michael Adler and Christof M. Niemeyer p. 293
Chapter 4.2 Rolling Circle Amplification in Multiplex Immunoassays Michael C. Mullenix and Richard S. Dondero and Hirock D. Datta and Michael Egholm and Stephen F. Kingsmore and Lorah T. Perlee p. 313
Index p. 333
DNA amplification : current technologies and applications /
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