2D PAGE : sample preparation and fractionation /

Preface p. v
Contributors p. xi
1 Application of Fluorescence Dye Saturation Labeling for Differential Proteome Analysis of 1,000 Microdissected Cells from Pancreatic Ductal Adenocarcinoma Precursor Lesions Barbara Sitek and Bence Sipos and Gunter Kloppel and Wolff Schmiegel and Stephan A. Hahn and Helmut E. Meyer and Kai Stuhler p. 1
2 Albumin and Immunoglobulin Depletion of Human Plasma Rosalind E. Jenkins and Neil R. Kitteringham and Carrie Greenough and B. Kevin Park p. 15
3 Multi-Component Immunoaffinity Subtraction and Reversed-Phase Chromatography of Human Serum James Martosella and Nina Zolotarjova p. 27
4 Immunoaffinity Fractionation of Plasma Proteins by Chicken IgY Antibodies Lei Huang and Xiangming Fang p. 41
5 Proteomics of Cerebrospinal Fluid: Methods for Sample Processing John E. Hale and Valentina Gelfanova and Jin-Sam You and Michael D. Knierman and Robert A. Dean p. 53
6 Sample Preparation of Bronchoalveolar Lavage Fluid Baptiste Leroy and Paul Falmagne and Ruddy Wattiez p. 67
7 Preparation of Nasal Secretions for Proteome Analysis Begona Casado and Paolo Iadarola and Lewis K. Pannell p. 77
8 Preparation of Urine Samples for Proteomic Analysis Rembert Pieper p. 89
9 Isolation of Cytoplasmatic Proteins from Cultured Cells for Two-Dimensional Gel Electrophoresis Ying Wang and Jen-Fu Chiu and Qing-Yu He p. 101
10 Sample Preparation of Culture Medium from Madin-Darby Canine Kidney Cells Daniel Ambort and Daniel Lottaz and Erwin Sterchi p. 113
11 Sample Preparation for Mass Spectrometry Analysis of Formalin-Fixed Paraffin-Embedded Tissue: Proteomic Analysis of Formalin-Fixed Tissue Nicolas A. Stewart and Timothy D. Veenstra p. 131
12 Metalloproteomics in the Molecular Study of Cell Physiology and Disease Hermann-Josef Thierse and Stefanie Helm and Patrick Pankert p. 139
13 Protein Extraction from Green Plant Tissue Ragnar Flengsrud p. 149
14 The Terminator: A Device for High-Throughput Extraction of Plant Material B. M. van den Berg p. 153
15 Isolation of Mitochondria from Plant Cell Culture Etienne H. Meyer and A. Harvey Millar p. 163
16 Isolation and Preparation of Chloroplasts from Arabidopsis thaliana Plants Sybille E. Kubis and Kathryn S. Lilley and Paul Jarvis p. 171
17 Isolation of Plant Cell Wall Proteins Elisabeth Jamet and Georges Boudart and Gisele Borderies and Stephane Charmont and Claude Lafitte and Michel Rossignol and Herve Canut and Rafael Pont-Lezica p. 187
18 Isolation and Fractionation of the Endoplasmic Reticulum from Castor Bean (Ricinus communis) Endosperm for Proteomic Analyses William J. Simon and Daniel J. Maltman and Antoni R. Slabas p. 203
19 Cell Wall Fractionation for Yeast and Fungal Proteomics Aida Pitarch and Cesar Nombela and Concha Gil p. 217
20 Collection of Proteins Secreted from Yeast Protoplasts in Active Cell Wall Regeneration Aida Pitarch and Cesar Nombela and Concha Gil p. 241
21 Sample Preparation Procedure for Cellular Fungi Alois Harder p. 265
22 Isolation and Enrichment of Secreted Proteins from Filamentous Fungi Martha L. Medina and Wilson A. Francisco p. 275
23 Isolation and Solubilization of Cellular Membrane Proteins from Bacteria Kheir Zuobi-Hasona and L. Jeannine Brady p. 287
24 Isolation and Solubilization of Gram-Positive Bacterial Cell Wall-Associated Proteins Jason N. Cole and Steven P. Djordjevic and Mark J. Walker p. 295
25 Cell Fractionation of Parasitic Protozoa Wanderley de Souza and Jose Andres Morgado-Diaz and Narcisa L. Cunha-e-Silva p. 313
Index p. 333