简介
Summary:
Publisher Summary 1
Kmiec, who discovered the technique known as "chimeraplasty" and currently works at Thomas Jefferson University in Pennsylvania, is joined by researchers from academia and the pharmaceutical industry to present the latest techniques for gene replacement, gene knockout, and gene repair in both plants and animals. They offer several methods for gene targeting, including Cre-ox, and adeno and adeno-associated viruses, as well as the new and potentially revolutionary use of oligonucleotides. This book will provide practical gene-targeting techniques for molecular and cell biologists, geneticists, and gene therapists, as well as biotechnologists involved in genetic manipulation. Annotation c. Book News, Inc., Portland, OR (booknews.com)
Publisher Summary 2
A panel of innovative investigators presents, in readily reproducible detail, the latest techniques for gene replacement, gene knockout, and gene repair in both plants and animals. These expert researchers review the best gene transfer vehicles (liposomes, PEI, and EPD), discuss the parameters that affect vector uptake, and detail successful methods for gene targeting (Cre-ox, and adeno and adeno-associated viruses). The methods include two fundamentally different approaches to gene targeting-one uses a fusion protein to deliver a vector to a virus for possible therapeutic effect, and the other entails an episomal-based Epstein-Barr vector that can modulate the chromatin assembly process, and thus overcome a serious barrier to therapeutic gene targeting. The book also has several techniques for using oligonucleotides in gene targeting, a methodology that may well revolutionize the entire field. Gene Targeting Protocols offers today's researchers robust and proven gene-targeting techniques that are essential to understanding biological processes at the genetic level.
目录
Preface p. v
Contributors p. xiii
1 Nucleic Acid Transfer Using Cationic Lipids Natasha J. Caplen p. 1
2 Optimizing Polyethylenimine-Based Gene Transfer into Mammalian Brain for Analysis of Promoter Regulation and Protein Function Barbara A. Demeneix and Mohamed Ghorbel and Daniel Goula p. 21
3 Gene Transfer and Drug Delivery by Electronic Pulse Delivery: A Nonviral Delivery Approach Xi Zhao p. 37
4 Strategies for Improving the Frequency and Assessment of Homologous Recombination Nancy Smyth Templeton p. 45
5 Effective Gene Transfer Using Viral Vectors Based on SV40 David S. Strayer p. 61
6 Rapid Generation of Isogenic Mammalian Cell Lines Expressing Recombinant Transgenes by Use of Cre Recombinase Bruce D. Bethke and Brian Sauer p. 75
7 Site-Directed Alteration of DNA by Small-Fragment Homologous Replacement Kaarin K. Goncz and Dieter C. Gruenert p. 85
8 Mutation Correction by Homologous Recombination with an Adenovirus VectorAyumi Fujita-Kusano Yasuhiro Naito and Izumu Saito and Ichizo Kobayashi p. 101
9 Site-Specific Targeting of DNA Plasmids to Chromosome 19Using AAV Cis and Trans Sequences Samuel M. Young Jr. and Weidong Xiao and Richard Jude Samulski p. 111
10 Adeno-Associated Virus Based Gene Therapy in Skeletal Muscle Richard J. Bartlett and Jesica M. McCue p. 127
11 Rapid Establishment of Myeloma Cell Lines Expressing Fab(Tac)-Protamine, a Targetable Protein Vector, Directed Against High-Affinity a-Chain of Human Interleukin-2 Receptor Sun U. Song and Wayne A. Marasco p. 157
12 EBV-Derived Episomes to Probe Chromatin Structure and Gene Expression in Human Cells Juliette Fivaz and M. Chiara Bassi and Stephane Pinaud and Larry Richman and Melanie Price and Jovan Mirkovitch p. 167
13 Triplex-Directed Site-Specific Genome Modification Karen M. Vasquez and John H. Wilson p. 183
14 Use of Quantitative Ligation-Mediated Polymerase Chain Reaction to Detect Gene Targeting by Alkylating Oligodeoxynucleotides Howard B. Gamper and Irina Afonina and Evgeniy Belousov and Michael W. Reed and Mikhail A. Podyminogin p. 201
15 Gene Targeting in Plants via Site-Directed Mutagenesis Peter B. Kipp and Joyce M. Van Eck and Peter R. Beetham and Gregory D. May p. 213
16 Antisense Oligonucleotides as Modulators of Pre-mRNA Splicing Halina Sierakowska and Sudhir Agrawal and Ryszard Kole p. 223
Index p. 235
Contributors p. xiii
1 Nucleic Acid Transfer Using Cationic Lipids Natasha J. Caplen p. 1
2 Optimizing Polyethylenimine-Based Gene Transfer into Mammalian Brain for Analysis of Promoter Regulation and Protein Function Barbara A. Demeneix and Mohamed Ghorbel and Daniel Goula p. 21
3 Gene Transfer and Drug Delivery by Electronic Pulse Delivery: A Nonviral Delivery Approach Xi Zhao p. 37
4 Strategies for Improving the Frequency and Assessment of Homologous Recombination Nancy Smyth Templeton p. 45
5 Effective Gene Transfer Using Viral Vectors Based on SV40 David S. Strayer p. 61
6 Rapid Generation of Isogenic Mammalian Cell Lines Expressing Recombinant Transgenes by Use of Cre Recombinase Bruce D. Bethke and Brian Sauer p. 75
7 Site-Directed Alteration of DNA by Small-Fragment Homologous Replacement Kaarin K. Goncz and Dieter C. Gruenert p. 85
8 Mutation Correction by Homologous Recombination with an Adenovirus VectorAyumi Fujita-Kusano Yasuhiro Naito and Izumu Saito and Ichizo Kobayashi p. 101
9 Site-Specific Targeting of DNA Plasmids to Chromosome 19Using AAV Cis and Trans Sequences Samuel M. Young Jr. and Weidong Xiao and Richard Jude Samulski p. 111
10 Adeno-Associated Virus Based Gene Therapy in Skeletal Muscle Richard J. Bartlett and Jesica M. McCue p. 127
11 Rapid Establishment of Myeloma Cell Lines Expressing Fab(Tac)-Protamine, a Targetable Protein Vector, Directed Against High-Affinity a-Chain of Human Interleukin-2 Receptor Sun U. Song and Wayne A. Marasco p. 157
12 EBV-Derived Episomes to Probe Chromatin Structure and Gene Expression in Human Cells Juliette Fivaz and M. Chiara Bassi and Stephane Pinaud and Larry Richman and Melanie Price and Jovan Mirkovitch p. 167
13 Triplex-Directed Site-Specific Genome Modification Karen M. Vasquez and John H. Wilson p. 183
14 Use of Quantitative Ligation-Mediated Polymerase Chain Reaction to Detect Gene Targeting by Alkylating Oligodeoxynucleotides Howard B. Gamper and Irina Afonina and Evgeniy Belousov and Michael W. Reed and Mikhail A. Podyminogin p. 201
15 Gene Targeting in Plants via Site-Directed Mutagenesis Peter B. Kipp and Joyce M. Van Eck and Peter R. Beetham and Gregory D. May p. 213
16 Antisense Oligonucleotides as Modulators of Pre-mRNA Splicing Halina Sierakowska and Sudhir Agrawal and Ryszard Kole p. 223
Index p. 235
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