Biocatalysts and enzyme technology /
副标题：无
作 者：Klaus Buchholz, Volker Kasche, Uwe Theo Bornscheuer.
分类号：
ISBN：9783527304974
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简介
"An instructive and comprehensive overview of our current knowledge of biocatalysis and enzyme technology. Following an introduction to the history of enzyme applications and the motivations for using these highly selective and environmentally friendly methods, the book goes on to cover enzyme mechanisms and kinetics, production, recovery, characterization and their design, including recombinant methods. Alongside the application of soluble and immobilized biocatalysts, including whole-cell systems, the authors treat the use of non-aqueous reaction systems, applications in organic synthesis, bioreactor design and reaction engineering. A number of case studies further exemplify the advantages of enzyme processes. Each topic includes exercises, designed to facilitate access to this flourishing area of research."--BOOK JACKET.
目录
Preface
Introduction to Enzyme Technology
Introduction
Goals and Potential of Biotechnological Production Processes
Historical Highlights of Enzyme Technology/Applied Biocatalysis
Biotechnological Processes: The Use of Isolated or Intracellular Enzymes as Biocatalysts
Advantages and Disadvantages of Enzyme-Based Production Processes
Goals, and Essential System Properties for New or Improved Enzyme Processes
Exercises
Basics of Enzymes as Biocatalysts
Introduction
Enzyme Classification
Enzyme Synthesis and Structure
Enzyme Function and its General Mechanism
Free Energy Changes and the Specificity of Enzyme-Catalyzed Reactions
Equilibrium- and Kinetically Controlled Reactions Catalyzed by Enzymes
Kinetics of Enzyme-Catalyzed Reactions
End-points of Enzyme Processes and Amount of Enzyme Required to Reach the Endpoint in a Given Time
Enzyme-catalyzed Processes with Slightly Soluble Products and Substrates
Stability, Denaturation, and Renaturation of Enzymes
Better Enzymes by Natural Evolution, in vitro Evolution, or Rational Enzyme Engineering
Exercises
Enzymes in Organic Chemistry
Introduction
Examples
Exercises
Enzyme Production and Purification
Introduction
Enzyme Sources
Improving Enzyme Yield
Increasing the Yield of Periplasmic and Extracellular Enzymes
Downstream Processing of Enzymes
Exercises
Application of Enzymes in Solution: Soluble Enzymes and Enzyme Systems
Introduction and Areas of Application
Space-Time-Yield and Productivity
Examples for the Application of Enzymes in Solution
Membrane Systems and Processes
Exercises
Immobilization of Enzymes (Including Applications)
Principles
Carriers
Binding Methods
Examples: Application of Immobilized Enzymes
Exercises
Immobilization of Microorganisms and Cells
Introduction
Immobilization by Aggregation/Flocculation
Immobilization by Entrapment
Adsorption
Adhesion
Outlook: Designed Cells
Exercises
Characterization of Immobilized Biocatalysts
Introduction
Factors Influencing the Space-Time Yield of Immobilized Biocatalysts
Effectiveness Factors for Immobilized Biocatalysts
Mass Transfer and Reaction
Space-Time Yields and Effectiveness Factors for Different Reactors
Determination of Essential Properties of Immobilized Biocatalysts
Comparison of Calculated and Experimental Data for Immobilized Biocatalysts
Application of Immobilized Biocatalysts for Enzyme Processes in Aqueous Suspensions
Improving the Performance of Immobilized Biocatalysts
Exercises
Reactors and Process Technology
Types of Reactors
Case Study 1: The Enzymatic Production of 7-ACA from Cephalosporin C
Residence Time Distribution, Mixing, Pressure Drop and Mass Transfer in Reactors
Process Technology
Exercises
The World of Biotechnology Information: Eight Points for Reflecting on Your Information Behavior
Thinking About Your Information Behavior
Playing with Databases and Search Terms
Tutorials, Subject Gateways and Literature Guides
Using Your Local Library
Using Encyclopedias
Searching Journal Articles, Patents, and Data
After Searching: Evaluating and Processing Information
Information and the World
Symbols
Subject Index
Introduction to Enzyme Technology
Introduction
Goals and Potential of Biotechnological Production Processes
Historical Highlights of Enzyme Technology/Applied Biocatalysis
Biotechnological Processes: The Use of Isolated or Intracellular Enzymes as Biocatalysts
Advantages and Disadvantages of Enzyme-Based Production Processes
Goals, and Essential System Properties for New or Improved Enzyme Processes
Exercises
Basics of Enzymes as Biocatalysts
Introduction
Enzyme Classification
Enzyme Synthesis and Structure
Enzyme Function and its General Mechanism
Free Energy Changes and the Specificity of Enzyme-Catalyzed Reactions
Equilibrium- and Kinetically Controlled Reactions Catalyzed by Enzymes
Kinetics of Enzyme-Catalyzed Reactions
End-points of Enzyme Processes and Amount of Enzyme Required to Reach the Endpoint in a Given Time
Enzyme-catalyzed Processes with Slightly Soluble Products and Substrates
Stability, Denaturation, and Renaturation of Enzymes
Better Enzymes by Natural Evolution, in vitro Evolution, or Rational Enzyme Engineering
Exercises
Enzymes in Organic Chemistry
Introduction
Examples
Exercises
Enzyme Production and Purification
Introduction
Enzyme Sources
Improving Enzyme Yield
Increasing the Yield of Periplasmic and Extracellular Enzymes
Downstream Processing of Enzymes
Exercises
Application of Enzymes in Solution: Soluble Enzymes and Enzyme Systems
Introduction and Areas of Application
Space-Time-Yield and Productivity
Examples for the Application of Enzymes in Solution
Membrane Systems and Processes
Exercises
Immobilization of Enzymes (Including Applications)
Principles
Carriers
Binding Methods
Examples: Application of Immobilized Enzymes
Exercises
Immobilization of Microorganisms and Cells
Introduction
Immobilization by Aggregation/Flocculation
Immobilization by Entrapment
Adsorption
Adhesion
Outlook: Designed Cells
Exercises
Characterization of Immobilized Biocatalysts
Introduction
Factors Influencing the Space-Time Yield of Immobilized Biocatalysts
Effectiveness Factors for Immobilized Biocatalysts
Mass Transfer and Reaction
Space-Time Yields and Effectiveness Factors for Different Reactors
Determination of Essential Properties of Immobilized Biocatalysts
Comparison of Calculated and Experimental Data for Immobilized Biocatalysts
Application of Immobilized Biocatalysts for Enzyme Processes in Aqueous Suspensions
Improving the Performance of Immobilized Biocatalysts
Exercises
Reactors and Process Technology
Types of Reactors
Case Study 1: The Enzymatic Production of 7-ACA from Cephalosporin C
Residence Time Distribution, Mixing, Pressure Drop and Mass Transfer in Reactors
Process Technology
Exercises
The World of Biotechnology Information: Eight Points for Reflecting on Your Information Behavior
Thinking About Your Information Behavior
Playing with Databases and Search Terms
Tutorials, Subject Gateways and Literature Guides
Using Your Local Library
Using Encyclopedias
Searching Journal Articles, Patents, and Data
After Searching: Evaluating and Processing Information
Information and the World
Symbols
Subject Index
Biocatalysts and enzyme technology /
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