Laser capture microscopy and microdissection /
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作 者:edited by P. Michael Conn.
分类号:
ISBN:9780121822590
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简介
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences. Major Sections Include: * Basic Principles, Specialized Uses, and Genetic Applications* LCM and its application in genomics and proteomics * Fluorescence in situ hybridization of LCM isolated nuclei from paraffin sections* Noncontact laser catapulting for the functional genomics and proteomics * Use of LCM for clonal analysis, in carcinoma analysis, to assess development, in complex tissue, in pathology, gene discovery, and more.
目录
Table Of Contents:
Contributors to Volume 356 ix
Preface xiii
Volumes in Series xv
Section I. Basic Principles
Comparison of Current Equipment 3(9)
Anda Cornea
Alison Mungenast
Laser Capture Microdissection and Its Applications in Genomics and Proteomics 12(13)
James L. Wittliff
Mark G. Erlander
Going in Vivo with Laser Microdissection 25(8)
Anette Mayer
Monika Stich
Dieter Brocksch
Karin Schutze
Georgia Lahr
Use of Laser Capture Microdissection to Selectively Obtain Distinct Populations of Cells for Proteomic Analysis 33(16)
Rachel A. Craven
Rosamonde E. Banks
Optimized Tissue Processing and Staining for Laser Capture Microdissection and Nucleic Acid Retrieval 49(14)
Lora E. Huang
Veronica Luzzi
Torsten Ehrig
Victoria Holtschlag
Mark A. Watson
Fluorescence in Situ Hybridization of LCM-Isolated Nuclei from Paraffin Sections 63(7)
Douglas J. Demetrick
Sabita K. Murthy
Lisa M. DiFrancesco
Immunoblotting of Single Cell Types Isolated from Frozen Sections by Laser Microdissection 70(10)
Livia Casciola-Rosen
Kanneboyina Nagaraju
Noncontact Laser Catapulting: A Basic Procedure for Functional Genomics and Proteomics 80(19)
Gabriela Westphal
Renate Burgemeister
Gabriele Friedemann
Axel Wellmann
Nicolas Wernert
Volker Wollscheid
Bernd Becker
Thomas Vogt
Ruth Knuchel
Wilhelm Stolz
Karin Schutze
Internal Standards for Laser Microdissection 99(15)
Ludger Fink
Rainer Maria Bohle
Methacarn: A Fixation Tool for Multipurpose Genetic Analysis from Paraffin-Embedded Tissues 114(15)
Makoto Shibutani
Chikako Uneyama
Section II. Specialized Uses
Use of Laser Capture Microdissection for Clonal Analysis 129(8)
Valerie Paradis
Pierre Bedossa
Laser Capture Microdissection in Carcinoma Analysis 137(8)
Yen-Li Lo
Chen-Yang Shen
Laser Capture Microdissection to Assess Development 145(12)
Carlos A. Suarez-Quian
Oscar M. Tirado
Francina Munell
Jaume Reventos
Application of Laser Capture Microdissection to Proteomics 157(11)
K. K. Jain
Laser Capture Microdissection of Mouse Intestine: Characterizing mRNA and Protein Expression, and Profiling Intermediary Metabolism in Specified Cell Populations 168(29)
Thaddeus S. Stappenbeck
Lora V. Hooper
Jill K. Manchester
Melissa H. Wong
Jeffrey I. Gordon
Laser Capture Microdissection in Pathology 197(11)
Falko Fend
Katja Specht
Marcus Kremer
Leticia Quintanilla-Martinez
Use of Laser Capture Microscopy in the Analysis of Mouse Models of Human Diseases 208(9)
Meral J. Arin
Dennis R. Roop
Use of Laser Microdissection in Complex Tissue 217(8)
Holger S. Willenberg
Rhodri Walters
Stefan R. Bornstein
Assessment of Clonal Relationships in Malignant Lymphomas 225(16)
Kojo S. J. Elenitoba-Johnson
Comparison of Normal and Tumor Cells by Laser Capture Microdissection 241(8)
Jaume Mora
Muzaffar Akram
William L. Gerald
Analysis of Folliculostellate Cells by Laser Capture Microdissection and Reverse Transcription--Polymerase Chain Reaction (LCM-RT/PCR) 249(10)
Ricardo V. Lloyd
Long Jin
Katharina H. Ruebel
Jill M. Bayliss
Section III. Genetic Applications
Analysis of Gene Expression 259(12)
Janette K. Burgess
Brent E. McParland
Analysis of Specific Gene Expression 271(11)
Georgia Lahr
Anna Starzinski-Powitz
Anette Mayer
Gene Discovery with Laser Capture Microscopy 282(7)
Mauricio Neira
Edwin Azen
DNA Fingerprinting from Cells Captured by Laser Microdissection 289(6)
Yongyut Sirivatanauksorn
Vorapan Sirivatanauksorn
Nicholas R. Lemoine
Single Cell PCR in Laser Capture Microscopy 295(7)
Sinuhe Hahn
Xiao Yan Zhong
Wolfgang Holzgreve
Assessment of Genetic Heterogeneity in Tumors Using Laser Capture Microdissection 302(7)
Dave S. B. Hoon
Akihide Fujimoto
Sherry Shu
Bret Taback
Gene Mutations: Analysis in Proliferative Prostatic Diseases Using Laser Capture Microdissection 309(14)
Hitoshi Takayama
Norio Nonomura
Katsuyuki Aozasa
Use of Laser Capture Microdissection-Generated Targets for Hybridization of High-Density Oligonucleotide Arrays 323(11)
Hiroe Ohyama
Mamatha Mahadevappa
Heikki Luukkaa
Randy Todd
Janet A. Warrington
David T. W. Wong
Single Cell Gene Mutation Analysis Using Laser-Assisted Microdissection of Tissue Sections 334(9)
Asa Persson
Helena Backvall
Fredrik Ponten
Mathias Uhlen
Joakim Lundeberg
Methylation in Gene Promoters: Assessment after Laser Capture Microdissection 343(10)
Arthur R. Brothman
Jiang Cui
Author Index 353(22)
Subject Index 375
Contributors to Volume 356 ix
Preface xiii
Volumes in Series xv
Section I. Basic Principles
Comparison of Current Equipment 3(9)
Anda Cornea
Alison Mungenast
Laser Capture Microdissection and Its Applications in Genomics and Proteomics 12(13)
James L. Wittliff
Mark G. Erlander
Going in Vivo with Laser Microdissection 25(8)
Anette Mayer
Monika Stich
Dieter Brocksch
Karin Schutze
Georgia Lahr
Use of Laser Capture Microdissection to Selectively Obtain Distinct Populations of Cells for Proteomic Analysis 33(16)
Rachel A. Craven
Rosamonde E. Banks
Optimized Tissue Processing and Staining for Laser Capture Microdissection and Nucleic Acid Retrieval 49(14)
Lora E. Huang
Veronica Luzzi
Torsten Ehrig
Victoria Holtschlag
Mark A. Watson
Fluorescence in Situ Hybridization of LCM-Isolated Nuclei from Paraffin Sections 63(7)
Douglas J. Demetrick
Sabita K. Murthy
Lisa M. DiFrancesco
Immunoblotting of Single Cell Types Isolated from Frozen Sections by Laser Microdissection 70(10)
Livia Casciola-Rosen
Kanneboyina Nagaraju
Noncontact Laser Catapulting: A Basic Procedure for Functional Genomics and Proteomics 80(19)
Gabriela Westphal
Renate Burgemeister
Gabriele Friedemann
Axel Wellmann
Nicolas Wernert
Volker Wollscheid
Bernd Becker
Thomas Vogt
Ruth Knuchel
Wilhelm Stolz
Karin Schutze
Internal Standards for Laser Microdissection 99(15)
Ludger Fink
Rainer Maria Bohle
Methacarn: A Fixation Tool for Multipurpose Genetic Analysis from Paraffin-Embedded Tissues 114(15)
Makoto Shibutani
Chikako Uneyama
Section II. Specialized Uses
Use of Laser Capture Microdissection for Clonal Analysis 129(8)
Valerie Paradis
Pierre Bedossa
Laser Capture Microdissection in Carcinoma Analysis 137(8)
Yen-Li Lo
Chen-Yang Shen
Laser Capture Microdissection to Assess Development 145(12)
Carlos A. Suarez-Quian
Oscar M. Tirado
Francina Munell
Jaume Reventos
Application of Laser Capture Microdissection to Proteomics 157(11)
K. K. Jain
Laser Capture Microdissection of Mouse Intestine: Characterizing mRNA and Protein Expression, and Profiling Intermediary Metabolism in Specified Cell Populations 168(29)
Thaddeus S. Stappenbeck
Lora V. Hooper
Jill K. Manchester
Melissa H. Wong
Jeffrey I. Gordon
Laser Capture Microdissection in Pathology 197(11)
Falko Fend
Katja Specht
Marcus Kremer
Leticia Quintanilla-Martinez
Use of Laser Capture Microscopy in the Analysis of Mouse Models of Human Diseases 208(9)
Meral J. Arin
Dennis R. Roop
Use of Laser Microdissection in Complex Tissue 217(8)
Holger S. Willenberg
Rhodri Walters
Stefan R. Bornstein
Assessment of Clonal Relationships in Malignant Lymphomas 225(16)
Kojo S. J. Elenitoba-Johnson
Comparison of Normal and Tumor Cells by Laser Capture Microdissection 241(8)
Jaume Mora
Muzaffar Akram
William L. Gerald
Analysis of Folliculostellate Cells by Laser Capture Microdissection and Reverse Transcription--Polymerase Chain Reaction (LCM-RT/PCR) 249(10)
Ricardo V. Lloyd
Long Jin
Katharina H. Ruebel
Jill M. Bayliss
Section III. Genetic Applications
Analysis of Gene Expression 259(12)
Janette K. Burgess
Brent E. McParland
Analysis of Specific Gene Expression 271(11)
Georgia Lahr
Anna Starzinski-Powitz
Anette Mayer
Gene Discovery with Laser Capture Microscopy 282(7)
Mauricio Neira
Edwin Azen
DNA Fingerprinting from Cells Captured by Laser Microdissection 289(6)
Yongyut Sirivatanauksorn
Vorapan Sirivatanauksorn
Nicholas R. Lemoine
Single Cell PCR in Laser Capture Microscopy 295(7)
Sinuhe Hahn
Xiao Yan Zhong
Wolfgang Holzgreve
Assessment of Genetic Heterogeneity in Tumors Using Laser Capture Microdissection 302(7)
Dave S. B. Hoon
Akihide Fujimoto
Sherry Shu
Bret Taback
Gene Mutations: Analysis in Proliferative Prostatic Diseases Using Laser Capture Microdissection 309(14)
Hitoshi Takayama
Norio Nonomura
Katsuyuki Aozasa
Use of Laser Capture Microdissection-Generated Targets for Hybridization of High-Density Oligonucleotide Arrays 323(11)
Hiroe Ohyama
Mamatha Mahadevappa
Heikki Luukkaa
Randy Todd
Janet A. Warrington
David T. W. Wong
Single Cell Gene Mutation Analysis Using Laser-Assisted Microdissection of Tissue Sections 334(9)
Asa Persson
Helena Backvall
Fredrik Ponten
Mathias Uhlen
Joakim Lundeberg
Methylation in Gene Promoters: Assessment after Laser Capture Microdissection 343(10)
Arthur R. Brothman
Jiang Cui
Author Index 353(22)
Subject Index 375
Laser capture microscopy and microdissection /
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