Laser capture microscopy and microdissection /

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作   者:edited by P. Michael Conn.

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ISBN:9780121822590

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简介

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences. Major Sections Include: * Basic Principles, Specialized Uses, and Genetic Applications* LCM and its application in genomics and proteomics * Fluorescence in situ hybridization of LCM isolated nuclei from paraffin sections* Noncontact laser catapulting for the functional genomics and proteomics * Use of LCM for clonal analysis, in carcinoma analysis, to assess development, in complex tissue, in pathology, gene discovery, and more.

目录

Table Of Contents:
Contributors to Volume 356 ix
Preface xiii
Volumes in Series xv

Section I. Basic Principles

Comparison of Current Equipment 3(9)

Anda Cornea

Alison Mungenast

Laser Capture Microdissection and Its Applications in Genomics and Proteomics 12(13)

James L. Wittliff

Mark G. Erlander

Going in Vivo with Laser Microdissection 25(8)

Anette Mayer

Monika Stich

Dieter Brocksch

Karin Schutze

Georgia Lahr

Use of Laser Capture Microdissection to Selectively Obtain Distinct Populations of Cells for Proteomic Analysis 33(16)

Rachel A. Craven

Rosamonde E. Banks

Optimized Tissue Processing and Staining for Laser Capture Microdissection and Nucleic Acid Retrieval 49(14)

Lora E. Huang

Veronica Luzzi

Torsten Ehrig

Victoria Holtschlag

Mark A. Watson

Fluorescence in Situ Hybridization of LCM-Isolated Nuclei from Paraffin Sections 63(7)

Douglas J. Demetrick

Sabita K. Murthy

Lisa M. DiFrancesco

Immunoblotting of Single Cell Types Isolated from Frozen Sections by Laser Microdissection 70(10)

Livia Casciola-Rosen

Kanneboyina Nagaraju

Noncontact Laser Catapulting: A Basic Procedure for Functional Genomics and Proteomics 80(19)

Gabriela Westphal

Renate Burgemeister

Gabriele Friedemann

Axel Wellmann

Nicolas Wernert

Volker Wollscheid

Bernd Becker

Thomas Vogt

Ruth Knuchel

Wilhelm Stolz

Karin Schutze

Internal Standards for Laser Microdissection 99(15)

Ludger Fink

Rainer Maria Bohle

Methacarn: A Fixation Tool for Multipurpose Genetic Analysis from Paraffin-Embedded Tissues 114(15)

Makoto Shibutani

Chikako Uneyama

Section II. Specialized Uses

Use of Laser Capture Microdissection for Clonal Analysis 129(8)

Valerie Paradis

Pierre Bedossa

Laser Capture Microdissection in Carcinoma Analysis 137(8)

Yen-Li Lo

Chen-Yang Shen

Laser Capture Microdissection to Assess Development 145(12)

Carlos A. Suarez-Quian

Oscar M. Tirado

Francina Munell

Jaume Reventos

Application of Laser Capture Microdissection to Proteomics 157(11)

K. K. Jain

Laser Capture Microdissection of Mouse Intestine: Characterizing mRNA and Protein Expression, and Profiling Intermediary Metabolism in Specified Cell Populations 168(29)

Thaddeus S. Stappenbeck

Lora V. Hooper

Jill K. Manchester

Melissa H. Wong

Jeffrey I. Gordon

Laser Capture Microdissection in Pathology 197(11)

Falko Fend

Katja Specht

Marcus Kremer

Leticia Quintanilla-Martinez

Use of Laser Capture Microscopy in the Analysis of Mouse Models of Human Diseases 208(9)

Meral J. Arin

Dennis R. Roop

Use of Laser Microdissection in Complex Tissue 217(8)

Holger S. Willenberg

Rhodri Walters

Stefan R. Bornstein

Assessment of Clonal Relationships in Malignant Lymphomas 225(16)

Kojo S. J. Elenitoba-Johnson

Comparison of Normal and Tumor Cells by Laser Capture Microdissection 241(8)

Jaume Mora

Muzaffar Akram

William L. Gerald

Analysis of Folliculostellate Cells by Laser Capture Microdissection and Reverse Transcription--Polymerase Chain Reaction (LCM-RT/PCR) 249(10)

Ricardo V. Lloyd

Long Jin

Katharina H. Ruebel

Jill M. Bayliss

Section III. Genetic Applications

Analysis of Gene Expression 259(12)

Janette K. Burgess

Brent E. McParland

Analysis of Specific Gene Expression 271(11)

Georgia Lahr

Anna Starzinski-Powitz

Anette Mayer

Gene Discovery with Laser Capture Microscopy 282(7)

Mauricio Neira

Edwin Azen

DNA Fingerprinting from Cells Captured by Laser Microdissection 289(6)

Yongyut Sirivatanauksorn

Vorapan Sirivatanauksorn

Nicholas R. Lemoine

Single Cell PCR in Laser Capture Microscopy 295(7)

Sinuhe Hahn

Xiao Yan Zhong

Wolfgang Holzgreve

Assessment of Genetic Heterogeneity in Tumors Using Laser Capture Microdissection 302(7)

Dave S. B. Hoon

Akihide Fujimoto

Sherry Shu

Bret Taback

Gene Mutations: Analysis in Proliferative Prostatic Diseases Using Laser Capture Microdissection 309(14)

Hitoshi Takayama

Norio Nonomura

Katsuyuki Aozasa

Use of Laser Capture Microdissection-Generated Targets for Hybridization of High-Density Oligonucleotide Arrays 323(11)

Hiroe Ohyama

Mamatha Mahadevappa

Heikki Luukkaa

Randy Todd

Janet A. Warrington

David T. W. Wong

Single Cell Gene Mutation Analysis Using Laser-Assisted Microdissection of Tissue Sections 334(9)

Asa Persson

Helena Backvall

Fredrik Ponten

Mathias Uhlen

Joakim Lundeberg

Methylation in Gene Promoters: Assessment after Laser Capture Microdissection 343(10)

Arthur R. Brothman

Jiang Cui
Author Index 353(22)
Subject Index 375

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