Working with animal chromosomes
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作 者：Herbert C. Macgregor, Jennifer M. Varley
分类号：
ISBN：9780471102953
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简介
A guide to handling giant polytene and lampbrush chromosomes, preparing chromatin, visualizing transcription, locating genes on chromosomes, identifing fine-scale polymorphisms on mitotic chromosomes, etc. Covers many specialized chromosome techniques; each method is tested and reproducible and comes with a model protocol. This 2nd Edition has several new sections on recently developed cytological techniques including spreading of synaptonemal complexes from cells in meiotic prophase, banding of mitotic chromosomes with Giemsa and fluorochromes, indirect immunofluorescence, lampbrush chromosomes from Xenopus laevis, and isolation of polytene chromosomes. Also covers new legislation affecting those who work with animal chromosomes.
目录
Chromosome Size, Number, and Shape
Mitotic Chromosomes
Chromosomes in Meiosis
Chromosome Banding
Polytene Chromosomes
Lampbrush Chromosomes
Visualization of Transcription
In Situ Hybridization
Autoradiography
Measuring Nuclear or Chromosomal DNA
Safety and the Law
References
Appendix: Names, Addresses and Telephone Numbers of Suppliers
Index
Mitotic Chromosomes
Chromosomes in Meiosis
Chromosome Banding
Polytene Chromosomes
Lampbrush Chromosomes
Visualization of Transcription
In Situ Hybridization
Autoradiography
Measuring Nuclear or Chromosomal DNA
Safety and the Law
References
Appendix: Names, Addresses and Telephone Numbers of Suppliers
Index
Working with animal chromosomes
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