Primary and stem cells : gene transfer technologies and applications /

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作者：edited by Uma Lakshmipathy, Bhaskar Thyagarajan.

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ISBN：9780470610749

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Summary: Publisher Summary 1 "This book describes basic cell engineering methods, emphasizing stem cell applications, and use of the genetically modified stem cells in cell therapy and drug discovery. Together, the chapters introduce and offer insights on new techniques for engineering of stem cells and the delivery of transgenes into stem cells via various viral and non-viral systems. The book offers a guide to the types of manipulations currently available to create genetically engineered stem cells that suit any investigator's purpose, whether it's basic science investigation, creation of disease models and screens, or cells for therapeutic applications"--Provided by publisher. Publisher Summary 2 This book describes basic cell engineering methods, emphasizing stem cell applications, and use of the genetically modified stem cells in cell therapy and drug discovery. Together, the chapters introduce and offer insights on new techniques for engineering of stem cells and the delivery of transgenes into stem cells via various viral and non-viral systems. The book offers a guide to the types of manipulations currently available to create genetically engineered stem cells that suit any investigator's purpose, whether it's basic science investigation, creation of disease models and screens, or cells for therapeutic applications.

Table Of Contents:
Foreword vii

Michele Calos
Preface ix
Contributors xi

PART I CLONING AND GENE DELIVERY 1(48)

1 DNA Assembly Technologies Based on Homologous Recombination 3(16)

Billyana Tsvetanova

Lansha Peng

Xiquan Liang

Ke Li

Jian-Ping Yang

Tony Ho

Josh Shirley

Liewei Xu

Jason Potter

Wieslaw Kudlicki

Todd Peterson

Federico Katzen

2 Multigene Assembly for Construction of Synthetic Operons: Creation and Delivery of an Optimized All-IN-One Expression Construct for Generating Mouse iPS Cells 19(18)

Takefumi Sone

Yoko Takata

Hiroe Kishine

Taichi Andoh

Fumio Imamoto

Jonathan D. Chesnut

3 Strategies for the Delivery of Naked DNA 37(12)

Jennifer C. Moore

Ronald P. Hart

PART II NONINTEGRATING TECHNOLOGIES 49(86)

4 Episomal Vectors 51(34)

Junying Yu

5 Nonintegrating DNA Virus 85(18)

Chris Kemp

David Kuninger

6 Nonintegrating RNA Viruses 103(16)

Noemi Fusaki

7 Protein Delivery 119(16)

Xiao-Jian Han

Kazuhito Tomizawa

PART III INTEGRATING TECHNOLOGIES 135(92)

8 Sleeping Beauty Transposon-Mediated Stable Gene Delivery 137(26)

Andrew Wilber

Xianzheng Zhou

Xin Huang

Dan S. Kaufman

R. Scott McIvor

9 Integrating Viral Vectors for Gene Modifications 163(36)

Gemma L. Mendel

Brian Paszkiet

Rafal P. Witek

10 Bacteriophage Integrases for Site-Specific Integration 199(12)

Bhaskar Thyagarajan

11 Improving Gene Targeting Efficiency in Human Pluripotent Stem Cells 211(16)

Sangyoon Han

Anna McCann

Louise C. Laurent

Jeanne F. Loring

Ying Liu

PART IV APPLICATIONS 227(46)

12 Modified Stem Cells as Disease Models and in Toxicology Screening 229(22)

Catharina Ellerstrom

Peter Sartipy

Raimund Strehl

Johan Hyllner

13 Screening and Drug Discovery 251(22)

Elizabeth A. Davenport

Robert S. Ames
Index 273

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