Recombinant DNA A short course
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ISBN:9780716714842
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简介
The third edition of the landmark text Recombinant DNA offers an authoritative, accessible, and engaging introduction to modern, genome centered biology from its foremost practitioners. The new edition explores core concepts in molecular biology in a contemporary inquiry based context, building its coverage around the most relevant and exciting ... more 籩xamples of current research and landmark experiments that redefined our understanding of DNA. As a result, students learn in a compelling way how working scientists make real high impact discoveries. The first chapters provide an introduction to the fundamental concepts of genetics and genomics, an inside look at the Human Genome Project, bioinformatic and experimental techniques for large scale genomic studies, and a survey of epigenetics and RNA interference. The final chapters cover the quest to identify disease causing genes, the genetic basis of cancer, and DNA fingerprinting and forensics. In these chapters the authors provide examples of practical applications in human medicine, and discuss the future of human genetics and genomics projects. ?less
目录
Establishing the role of genes within cells
DNA is the primary genetic material
Elucidation of the genetic code
The genetic elements that control gene expression
Methods of creating recombinant DNA molecules
The isolation of cloned genes
The unxpected complexity of eukaryotic genes
In vitro mutagenesis
Rearranging germ-line DNA segments to form antibody genes
Tumor viruses
Movable genes
The experimentally controlled introduction of DNA into yeast cells
Genetic engineering of plants by using crown gall plasmids
Transferring genes into mammalian cells
Viral vectors
The introduction of foreign genes into fertilized mouse eggs
Recombinant DNA and genetic diseases
The science used in the recombinant DNA industry
Recombinant DNA dateline
Appendix A: Restriction enzymes
Appendix B: Other enzymes used in recombinant DNA research.
DNA is the primary genetic material
Elucidation of the genetic code
The genetic elements that control gene expression
Methods of creating recombinant DNA molecules
The isolation of cloned genes
The unxpected complexity of eukaryotic genes
In vitro mutagenesis
Rearranging germ-line DNA segments to form antibody genes
Tumor viruses
Movable genes
The experimentally controlled introduction of DNA into yeast cells
Genetic engineering of plants by using crown gall plasmids
Transferring genes into mammalian cells
Viral vectors
The introduction of foreign genes into fertilized mouse eggs
Recombinant DNA and genetic diseases
The science used in the recombinant DNA industry
Recombinant DNA dateline
Appendix A: Restriction enzymes
Appendix B: Other enzymes used in recombinant DNA research.
Recombinant DNA A short course
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